Gellissen G, Melber K, Janowicz Z A, Dahlems U M, Weydemann U, Piontek M, Strasser A W, Hollenberg C P
Rhein Biotech GmbH, Düsseldorf, Germany.
Antonie Van Leeuwenhoek. 1992 Aug;62(1-2):79-93. doi: 10.1007/BF00584464.
The exploitation of recombinant DNA technology to engineer expression systems for heterologous proteins represented a major task within the field of biotechnology during the last decade. Yeasts attracted the attention of molecular biologists because of properties most favourable for their use as hosts in heterologous protein production. Yeasts follow the general eukaryotic posttranslational modification pattern of expressed polypeptides, exhibit the ability to secrete heterologous proteins and benefit from an established fermentation technology. Aside from the baker's yeast Saccharomyces cerevisiae, an increasing number of alternative non-Saccharomyces yeast species are used as expression systems in basic research and for an industrial application. In the following review a selection from the different yeast systems is described and compared.
在过去十年中，利用重组DNA技术构建异源蛋白表达系统是生物技术领域的一项主要任务。酵母因其具有作为异源蛋白生产宿主的最有利特性而吸引了分子生物学家的关注。酵母遵循表达多肽的一般真核生物翻译后修饰模式，具有分泌异源蛋白的能力，并受益于成熟的发酵技术。除了酿酒酵母外，越来越多的其他非酿酒酵母物种被用作基础研究和工业应用中的表达系统。在以下综述中，将对不同酵母系统进行选择描述和比较。
