采用无标记探针和全长扩增子的同时高分辨率熔解分析对 aac(6')-Ib-cr 基因进行分型。
aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon.
机构信息
SA Pathology, Women's and Children's Hospital, 72 King William Rd., North Adelaide, SA 5006, Australia.
出版信息
Antimicrob Agents Chemother. 2010 Mar;54(3):1378-80. doi: 10.1128/AAC.01476-09. Epub 2009 Dec 14.
Abstract
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
摘要
我们开发了一种耗时和成本效益高的一步封闭管测定法，用于对 aac(6')-Ib-cr 进行基因分型，能够区分两种遗传 aac(6')-Ib-cr 变体。我们的基因分型测定法利用未标记探针同时获得的高分辨率熔化数据和全长扩增子的综合信息。
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