Totsuka A, Ohtaki K, Tagaya I
J Gen Virol. 1978 Mar;38(3):519-33. doi: 10.1099/0022-1317-38-3-519.
Virion aggregation in low ionic conditions was observed with small plaque variants of Coxsackievirus type B3 and Echovirus types 4 and 11 by sedimentation and filtration methods. Inclusion of salts or DEAE-dextran into the media prevented or reversed virion aggregation. The effect of pH on aggregate formation in low ionic strength solutions was also investigated with various strains of poliovirus. Type I Sabin strain formed aggregates even at high pH, while Mahoney strains did so only below pH 6.5. Type 2 virus, Sabin and MEF1 strains, and type 3 virus, Sabin, Saukett and Suwa strains, showed an intermediate behaviour between the two type 1 strains, except MEF1-LB strain, a clone obtained from MEF1 strain under acidic overlay, which showed little tendency to aggregate. These results were compared with the degree of the d character of the strains. Besides the effect of inhibiting virion aggregation, the inclusion of DEAE-dextran into a sucrose gradient slowed the sedimentation of some of the viruses in low ionic strength solutions.
通过沉降和过滤方法，在低离子强度条件下观察到B3型柯萨奇病毒以及4型和11型艾柯病毒的小蚀斑变种的病毒粒子聚集现象。向培养基中添加盐或葡聚糖硫酸酯可防止或逆转病毒粒子聚集。还使用多种脊髓灰质炎病毒株研究了pH对低离子强度溶液中聚集体形成的影响。I型萨宾株即使在高pH值下也会形成聚集体，而马奥尼株仅在pH值低于6.5时才会形成聚集体。2型病毒的萨宾株和MEF1株，以及3型病毒的萨宾株、索基特株和诹访株，表现出介于两种1型株之间的中间行为，但从MEF1株在酸性覆盖下获得的克隆MEF1-LB株几乎没有聚集倾向。将这些结果与各毒株的d特征程度进行了比较。除了抑制病毒粒子聚集的作用外，在蔗糖梯度中添加葡聚糖硫酸酯会减缓低离子强度溶液中一些病毒的沉降。
