Jensen H, Thomas K, Sharp D G
Appl Environ Microbiol. 1980 Sep;40(3):633-40. doi: 10.1128/aem.40.3.633-640.1980.
The inactivation rates of coxsackievirus B3 (CB3) and B5 (CB5) by chlorine in dilute buffer at pH 6 were very nearly the same and about half that of poliovirus (Mahoney) under similar conditions. Purified CB3, like the poliovirus, aggregated in the acid range but not at pH 7 and above. Purified CB5 aggregated rapidly at all pH values; still, the graph of log surviving infectivity versus time was a straight line. No chlorine inactivation data were obtained with dispersed CB5, for it could be dispersed only by addition of diethylaminoethyl dextran, which would react with the chlorine. Addition of 0.1 M NaCl to the buffer at pH 6 did not influence the aggregation of CB5 or the rate of chlorine action on either of the coxsackie-viruses, but at pH 10 it increased the disinfection activity of OCl- for both viruses roughly 20-fold. Cesium chloride had a similar but smaller effect. KCl was the most active of the three in this respect, making the inactivating effect of OCl- at pH 10 about equal to that of HOCl at pH 6.
在pH值为6的稀缓冲液中，柯萨奇病毒B3（CB3）和B5（CB5）被氯灭活的速率非常接近，在类似条件下约为脊髓灰质炎病毒（马奥尼株）的一半。纯化的CB3与脊髓灰质炎病毒一样，在酸性范围内会聚集，但在pH值为7及以上时不会聚集。纯化的CB5在所有pH值下都会迅速聚集；不过，对数存活感染力与时间的关系图呈直线。对于分散的CB5，未获得氯灭活数据，因为它只能通过添加二乙氨基乙基葡聚糖来分散，而二乙氨基乙基葡聚糖会与氯发生反应。在pH值为6的缓冲液中添加0.1 M NaCl不会影响CB5的聚集或氯对任何一种柯萨奇病毒的作用速率，但在pH值为10时，它会使OCl-对两种病毒的消毒活性大致提高20倍。氯化铯有类似但较小的影响。在这方面，KCl是三者中活性最高的，使OCl-在pH值为10时的灭活效果与HOCl在pH值为6时的效果相当。
