Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA.
Curr Genomics. 2010 Mar;11(1):40-6. doi: 10.2174/138920210790218016.
Protein-protein interaction network represents an important aspect of systems biology. The understanding of the plant protein-protein interaction network and interactome will provide crucial insights into the regulation of plant developmental, physiological, and pathological processes. In this review, we will first define the concept of plant interactome and the protein-protein interaction network. The significance of the plant interactome study will be discussed. We will then compare the pros and cons for different strategies for interactome mapping including yeast two-hybrid system (Y2H), affinity purification mass spectrometry (AP-MS), bimolecular fluorescence complementation (BiFC), and in silico prediction. The application of these platforms on specific plant biology questions will be further discussed. The recent advancements revealed the great potential for plant protein-protein interaction network and interactome to elucidate molecular mechanisms for signal transduction, stress responses, cell cycle control, pattern formation, and others. Mapping the plant interactome in model species will provide important guideline for the future study of plant biology.
蛋白质-蛋白质相互作用网络代表了系统生物学的一个重要方面。理解植物蛋白质-蛋白质相互作用网络和相互作用组将为植物发育、生理和病理过程的调控提供关键的见解。在这篇综述中，我们首先将定义植物相互作用组和蛋白质-蛋白质相互作用网络的概念。我们将讨论植物相互作用组研究的意义。然后，我们将比较不同相互作用组图谱策略的优缺点，包括酵母双杂交系统（Y2H）、亲和纯化质谱（AP-MS）、双分子荧光互补（BiFC）和计算预测。我们将进一步讨论这些平台在特定植物生物学问题上的应用。最近的进展揭示了植物蛋白质-蛋白质相互作用网络和相互作用组阐明信号转导、应激反应、细胞周期控制、形态发生等分子机制的巨大潜力。在模式物种中绘制相互作用组图谱将为植物生物学的未来研究提供重要的指导。
