Mellors R C, Mellors J W
Proc Natl Acad Sci U S A. 1978 May;75(5):2463-7. doi: 10.1073/pnas.75.5.2463.
Postmortem study of proliferative glomerulonephritis associated with human systemic lumpus has previously shown that an antigen related to mammalian type C RNA viral core (p30) proteins is deposited in the renal glomerular lesions with human immunoglobulins in an immune-complex pattern. In the present work, human immunoglobulins were sequentially eluted from the lupus glomerular immune deposits and were assayed by a sensitive enzymoimmunoassay developed for the measurement of anti-p30 antibody activity against purified viral p30 proteins of mammalian type C viruses. Human immunoglobulins showing specific anti-p30 antibody activity, particularly against p30 antigen of feline endogenous virus RD-114 and to a smaller extent against p30 antigen of murine type C virus, were eluted by acid buffer from the glomerular immune deposits in two patients with lupus proliferative glomerulonephritis who have deposits of viral p30-related antigen in the same tissue lesions. This study adds support for the hypothesis that expression of type C viral antigen may be involved in the multifactorial pathogenesis of proliferative glomerulonephritis associated with human systemic lupus.
先前对与人类系统性红斑狼疮相关的增殖性肾小球肾炎进行的尸检研究表明，一种与哺乳动物C型RNA病毒核心（p30）蛋白相关的抗原以免疫复合物形式与人类免疫球蛋白一起沉积在肾小球病变中。在本研究中，从狼疮性肾小球免疫沉积物中依次洗脱人类免疫球蛋白，并通过一种灵敏的酶免疫测定法进行检测，该方法用于测量针对哺乳动物C型病毒纯化病毒p30蛋白的抗p30抗体活性。在两名患有狼疮性增殖性肾小球肾炎且在相同组织病变中有病毒p30相关抗原沉积的患者中，用酸性缓冲液从肾小球免疫沉积物中洗脱显示出特异性抗p30抗体活性的人类免疫球蛋白，尤其是针对猫内源性病毒RD - 114的p30抗原，在较小程度上针对鼠C型病毒的p30抗原。这项研究为C型病毒抗原的表达可能参与与人类系统性红斑狼疮相关的增殖性肾小球肾炎的多因素发病机制这一假说提供了支持。
