Leiss Kirsten A, Choi Young H, Verpoorte Robert, Klinkhamer Peter G L
Section Plant Ecology and Metabolomics, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.
Phytochem Rev. 2011 Jun;10(2):205-216. doi: 10.1007/s11101-010-9175-z. Epub 2010 Apr 3.
Secondary metabolites provide a potential source for the generation of host plant resistance and development of biopesticides. This is especially important in view of the rapid and vast spread of agricultural and horticultural pests worldwide. Multiple pests control tactics in the framework of an integrated pest management (IPM) programme are necessary. One important strategy of IPM is the use of chemical host plant resistance. Up to now the study of chemical host plant resistance has, for technical reasons, been restricted to the identification of single compounds applying specific chemical analyses adapted to the compound in question. In biological processes however, usually more than one compound is involved. Metabolomics allows the simultaneous detection of a wide range of compounds, providing an immediate image of the metabolome of a plant. One of the most universally used metabolomic approaches comprises nuclear magnetic resonance spectroscopy (NMR). It has been NMR which has been applied as a proof of principle to show that metabolomics can constitute a major advancement in the study of host plant resistance. Here we give an overview on the application of NMR to identify candidate compounds for host plant resistance. We focus on host plant resistance to western flower thrips (Frankliniella occidentalis) which has been used as a model for different plant species.
次生代谢产物为宿主植物抗性的产生和生物农药的开发提供了潜在来源。鉴于农业和园艺害虫在全球范围内迅速广泛传播，这一点尤为重要。在综合虫害管理（IPM）计划框架内采用多种害虫控制策略是必要的。IPM的一项重要策略是利用化学宿主植物抗性。到目前为止，由于技术原因，化学宿主植物抗性的研究仅限于通过适用于特定化合物的特定化学分析来鉴定单一化合物。然而，在生物过程中，通常涉及不止一种化合物。代谢组学能够同时检测多种化合物，提供植物代谢组的即时图像。最普遍使用的代谢组学方法之一包括核磁共振光谱（NMR）。正是NMR被用作原理证明，以表明代谢组学可以在宿主植物抗性研究中取得重大进展。在此，我们概述了NMR在鉴定宿主植物抗性候选化合物方面的应用。我们重点关注宿主植物对西花蓟马（Frankliniella occidentalis）的抗性，西花蓟马已被用作不同植物物种的模型。
