Hejkal T W, Wellings F M, LaRock P A, Lewis A L
Appl Environ Microbiol. 1979 Jul;38(1):114-8. doi: 10.1128/aem.38.1.114-118.1979.
Poliovirus in fecal homogenates was used to determine the protection against inactivation by chlorination afforded virus that was occluded within particulates. Virus that was closely associated with or occluded within small fecal particulates was protected. A fourfold increase in combined residual chlorine was required to achieve the same degree of inactivation for occluded virus as for free or secondarily adsorbed virus. A combined chlorine residual of 6.6 mg/liter was necessary to achieve 50% inactivation in 15 min at pH 8.0 and 22 degrees C in a particulate suspension containing occluded virus compared to 1.4 mg/liter for free virus. These differences were found to be relatively small compared to differences due to the presence of dissolved organics or between free and combined chlorine residuals. The results suggest different mechanisms of protection due to adsorption and occlusion.
使用粪便匀浆中的脊髓灰质炎病毒来确定被包裹在颗粒中的病毒对氯化灭活的抵抗力。与小粪便颗粒紧密相关或被包裹在其中的病毒受到保护。对于被包裹的病毒，要达到与游离或二次吸附病毒相同程度的灭活，所需的总余氯量要增加四倍。在含有被包裹病毒的颗粒悬浮液中，在pH 8.0和22摄氏度下，要在15分钟内实现50%的灭活，总氯残留量需要达到6.6毫克/升，而游离病毒则需要1.4毫克/升。与由于溶解有机物的存在或游离氯与总氯残留之间的差异相比，这些差异相对较小。结果表明，由于吸附和包裹存在不同的保护机制。
