Key Laboratory of Coastal Environmental Processes, Chinese Academy of Sciences, Yantai, China.
World J Microbiol Biotechnol. 2012 Feb;28(2):447-52. doi: 10.1007/s11274-011-0834-z. Epub 2011 Jul 9.
With the intensive application of sulfonamides in aquaculture and animal husbandry and the increase of sulfonamides discharged into the environments, there is an increasing need to find a way to remediate sulfonamide-contaminated environments. Two bacterial strains capable of degrading sulfonamides, HS21 and HS51, were isolated from marine environments. HS21 and HS51 were identified as members of Escherichia sp. and Acinetobacter sp., respectively, based on 16S rRNA gene sequencing. Degradation of each sulfonamide by Escherichia sp. HS21 and Acinetobacter sp. HS51 was characterized using capillary electrophoresis. About 66 or 72% of sulfapyridine and 45 or 67% of sulfathiazole contained in the media was degraded by Escherichia sp. HS21 or Acinetobacter sp. HS51, respectively, after incubation for 2 days. The supernatant from culture of Escherichia sp. HS21 or Acinetobacter sp. HS51 grown in sulfapyridine or sulfathiazole contained media had much attenuated cytotoxicity against HeLa cells. These results suggest that Escherichia sp. HS21 and Acinetobacter sp. HS51 are new bacterial resources for biodegrading sulfonamides and indicate the potential of isolated strains for the bioremediation of sulfonamide-polluted environments.
随着磺胺类药物在水产养殖和畜牧业中的广泛应用以及磺胺类药物排放到环境中的增加，人们越来越需要寻找一种方法来修复受磺胺类药物污染的环境。从海洋环境中分离到了两株能够降解磺胺类药物的细菌菌株 HS21 和 HS51。根据 16S rRNA 基因测序，HS21 和 HS51 分别被鉴定为大肠杆菌和不动杆菌属的成员。使用毛细管电泳法对大肠杆菌 HS21 和不动杆菌属 HS51 对每种磺胺类药物的降解进行了表征。经过 2 天的孵育，大肠杆菌 HS21 或不动杆菌属 HS51 可分别降解培养基中 66%或 72%的磺胺吡啶和 45%或 67%的磺胺噻唑。在含有磺胺吡啶或磺胺噻唑的培养基中生长的大肠杆菌 HS21 或不动杆菌属 HS51 的培养上清液对 HeLa 细胞的细胞毒性明显减弱。这些结果表明，大肠杆菌 HS21 和不动杆菌属 HS51 是降解磺胺类药物的新细菌资源，并表明分离株在磺胺类药物污染环境的生物修复中的潜力。
