College of Plant Sciences, Jilin University, Changchun, China.
Extremophiles. 2013 Mar;17(2):193-203. doi: 10.1007/s00792-012-0509-1. Epub 2013 Jan 3.
Enzymes from many archaea colonizing extreme environments are of great interest because of their potential for various biotechnological processes and scientific value of evolution. Many enzymes from archaea have been reported to catalyze promiscuous reactions or moonlight in different functions. Here, we summarize known archaeal enzymes of both groups that include different kinds of proteins. Knowledge of their biochemical properties and three-dimensional structures has proved invaluable in understanding mechanism, application, and evolutionary implications of this manifestation. In addition, the review also summarizes the methods to unravel the extra function which almost was discovered serendipitously. The study of these amazing enzymes will provide clues to optimize protein engineering applications and how enzymes might have evolved on Earth.
由于其在各种生物技术过程中的潜在应用和进化的科学价值，来自极端环境的许多古菌的酶类引起了人们的极大兴趣。已经有报道称，许多古菌酶能够催化混杂反应或在不同功能中兼职。在这里，我们总结了这两类包括不同种类蛋白质的已知古菌酶。它们的生化特性和三维结构的知识对于理解这种表现形式的机制、应用和进化意义非常有价值。此外，该综述还总结了揭示这种几乎偶然发现的额外功能的方法。对这些神奇酶的研究将为优化蛋白质工程应用以及酶类在地球上是如何进化的提供线索。
