在活体组织中进行 FRET 激发的比率氧传感。
FRET excited ratiometric oxygen sensing in living tissue.
机构信息
Center for Neural Engineering, Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA, USA.
出版信息
J Neurosci Methods. 2013 Mar 30;214(1):45-51. doi: 10.1016/j.jneumeth.2013.01.002. Epub 2013 Jan 17.
Abstract
Dynamic analysis of oxygen (O₂) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O₂] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.
摘要
氧（O₂）的动态分析受到缺乏实时、定量和生物相容性传感器的限制。为了解决这些需求，我们设计了一种比率型光导矩阵，由磷光猝灭染料铂（II）辛基卟啉酮（PtOEPK）和纳米晶体量子点（NQDs）组成，当嵌入惰性聚合物基质中时，允许通过荧光共振能量转移（FRET）进行长期预设计激发。将该基质沉积在各种玻璃基底上，开发了一系列光学传感器，能够以数百毫秒的时间分辨率测量活跃脑组织的不同生物微域中的间质氧浓度[O₂]。
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