Thomas I, Brochier B, Languet B, Blancou J, Peharpre D, Kieny M P, Desmettre P, Chappuis G, Pastoret P P
Department of Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
J Gen Virol. 1990 Jan;71 ( Pt 1):37-42. doi: 10.1099/0022-1317-71-1-37.
The primary multiplication site of VVTGgRAB, a recombinant vaccinia virus (VV) expressing the rabies virus G glycoprotein, was studied in comparison with that of the parental VV Copenhagen strain, after oral administration to foxes. Foxes were fed with 10(8) TCID50 of either VVTGgRAB or VV and were sacrificed 12, 24, 48 or 96 h after inoculation. Both viruses were detected by viral isolation in the tonsils during the first 48 h after inoculation at titres between 10(2) and 10(4.3) TCID50/ml. Indirect immunofluorescence confirmed the presence of the virus in tonsils of some of the foxes. The polymerase chain reaction allowed the detection of VVTGgRAB in the tonsils of both of two foxes tested after 24 h, three of three foxes after 48 h, in the buccal mucosa of one of two foxes tested after 24 h and two of three foxes after 48 h and in the soft palate of one of two foxes tested after 24 h and one of three foxes after 48 h. VV was detected in the tonsils of one fox tested after 48 h, in the buccal mucosa of another fox tested after 24 h, and in the first fox after 48 h by the same reaction. Foxes were inoculated with virus isolated from fox tonsils 24 h after oral administration (with or without cell culture amplification) to perform back passages. No virus could be isolated in either case after this passage. The innocuity of VVTGgRAB was also demonstrated when foxes were inoculated with passaged virus.
在给狐狸口服接种后，对表达狂犬病病毒G糖蛋白的重组痘苗病毒（VV）VVTGgRAB的主要增殖部位与亲本VV哥本哈根株的主要增殖部位进行了比较研究。给狐狸喂食10⁸ TCID₅₀的VVTGgRAB或VV，并在接种后12、24、48或96小时处死。在接种后的头48小时内，通过病毒分离在扁桃体中检测到两种病毒，滴度在10²至10⁴.³ TCID₅₀/ml之间。间接免疫荧光证实了部分狐狸扁桃体中存在病毒。聚合酶链反应可在接种24小时后检测的两只狐狸中的两只、48小时后检测的三只狐狸中的三只、24小时后检测的两只狐狸中的一只的颊黏膜、48小时后检测的三只狐狸中的两只以及24小时后检测的两只狐狸中的一只和48小时后检测的三只狐狸中的一只的软腭中检测到VVTGgRAB。通过相同反应在48小时后检测的一只狐狸的扁桃体、24小时后检测的另一只狐狸的颊黏膜以及48小时后的第一只狐狸中检测到VV。在口服接种后24小时（有无细胞培养扩增）从狐狸扁桃体中分离出病毒接种狐狸以进行传代。在这一传代后，两种情况下均未分离到病毒。当用传代病毒接种狐狸时，也证明了VVTGgRAB的无害性。
