Liu Yi, Yang Ruili, Shi Songtao
1 Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing, China .
Tissue Eng Part A. 2015 Feb;21(3-4):498-509. doi: 10.1089/ten.TEA.2013.0673. Epub 2015 Jan 19.
Mesenchymal-stem-cell-based regenerative medicine is a promising approach for functional tissue reconstruction. A recent study showed that host immune cells regulated bone marrow mesenchymal stem cell (BMMSC)-mediated tissue regeneration. However, it is unknown whether systemic infusion of BMMSCs, which induces immune tolerance, affects cell-based tissue regeneration. In this study, we showed that BMMSCs possessed an immunomodulatory function in vitro. Moreover, systemic infusion of BMMSCs reduced IFN-γ and TNF-α levels in the implantation sites via upregulation of regulatory T cells (Tregs), resulting in marked enhancement of cell-based bone regeneration, but with only limited contribution by BMMSC homing. Further, we showed that systemic BMMSC infusion significantly improved cell-based repair of critical-sized calvarial defects in a murine model. These results suggested a new approach to enhance cell-based bone regeneration.
基于间充质干细胞的再生医学是一种用于功能性组织重建的很有前景的方法。最近一项研究表明，宿主免疫细胞可调节骨髓间充质干细胞（BMMSC）介导的组织再生。然而，诱导免疫耐受的BMMSCs全身输注是否会影响基于细胞的组织再生尚不清楚。在本研究中，我们表明BMMSCs在体外具有免疫调节功能。此外，BMMSCs的全身输注通过上调调节性T细胞（Tregs）降低了植入部位的IFN-γ和TNF-α水平，从而显著增强了基于细胞的骨再生，但BMMSC归巢的贡献有限。此外，我们表明全身输注BMMSCs可显著改善小鼠模型中临界尺寸颅骨缺损的基于细胞的修复。这些结果提示了一种增强基于细胞的骨再生的新方法。
