前列腺切除术前饮用绿茶和红茶对前列腺癌男性患者影响的随机临床试验。
Randomized clinical trial of brewed green and black tea in men with prostate cancer prior to prostatectomy.
作者信息
Henning Susanne M, Wang Piwen, Said Jonathan W, Huang Min, Grogan Tristan, Elashoff David, Carpenter Catherine L, Heber David, Aronson William J
机构信息
Center for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, CA.
出版信息
Prostate. 2015 Apr 1;75(5):550-9. doi: 10.1002/pros.22943. Epub 2014 Dec 24.
BACKGROUND
Preclinical and epidemiologic studies suggest chemopreventive effects of green tea (GT) and black tea (BT) in prostate cancer. In the current study we determined the effect of GT and BT consumption on biomarkers related to prostate cancer development and progression.
METHODS
In this exploratory, open label, phase II trial 113 men diagnosed with prostate cancer were randomized to consume six cups daily of brewed GT, BT or water (control) prior to radical prostatectomy (RP). The primary endpoint was prostate tumor markers of cancer development and progression determined by tissue immunostaining of proliferation (Ki67), apoptosis (Bcl-2, Bax, Tunel), inflammation (nuclear and cytoplasmic nuclear factor kappa B [NFκB]) and oxidation (8-hydroxydeoxy-guanosine [8OHdG]). Secondary endpoints of urinary oxidation, tea polyphenol uptake in prostate tissue, and serum prostate specific antigen (PSA) were evaluated by high performance liquid chromatography and ELISA analysis.
RESULTS
Ninety three patients completed the intervention. There was no significant difference in markers of proliferation, apoptosis and oxidation in RP tissue comparing GT and BT to water control. Nuclear staining of NFκB was significantly decreased in RP tissue of men consuming GT (P = 0.013) but not BT (P = 0.931) compared to water control. Tea polyphenols were detected in prostate tissue from 32 of 34 men consuming GT but not in the other groups. Evidence of a systemic antioxidant effect was observed (reduced urinary 8OHdG) only with GT consumption (P = 0.03). GT, but not BT or water, also led to a small but statistically significant decrease in serum prostate-specific antigen (PSA) levels (P = 0.04).
CONCLUSION
Given the GT-induced changes in NFκB and systemic oxidation, and uptake of GT polyphenols in prostate tissue, future longer-term studies are warranted to further examine the role of GT for prostate cancer prevention and treatment, and possibly for other prostate conditions such as prostatitis.
背景
临床前和流行病学研究表明绿茶（GT）和红茶（BT）对前列腺癌具有化学预防作用。在本研究中，我们确定了饮用GT和BT对与前列腺癌发生和进展相关生物标志物的影响。
方法
在这项探索性、开放标签的II期试验中，113名被诊断为前列腺癌的男性在根治性前列腺切除术（RP）前被随机分配，每天饮用六杯冲泡的GT、BT或水（对照）。主要终点是通过对增殖（Ki67）、凋亡（Bcl-2、Bax、Tunel）、炎症（核和细胞质核因子κB [NFκB]）和氧化（8-羟基脱氧鸟苷 [8OHdG]）进行组织免疫染色来确定的前列腺癌发生和进展的肿瘤标志物。通过高效液相色谱和ELISA分析评估尿液氧化、前列腺组织中茶多酚摄取以及血清前列腺特异性抗原（PSA）等次要终点。
结果
93名患者完成了干预。与水对照相比，在RP组织中，GT和BT组在增殖、凋亡和氧化标志物方面与水对照组无显著差异。与水对照相比，饮用GT的男性RP组织中NFκB的核染色显著降低（P = 0.013），而饮用BT的男性则无显著降低（P = 0.931）。在饮用GT的34名男性中的32名的前列腺组织中检测到了茶多酚，而其他组未检测到。仅饮用GT时观察到全身抗氧化作用的证据（尿液8OHdG降低）（P = 0.03）。GT而非BT或水还导致血清前列腺特异性抗原（PSA）水平出现虽小但具有统计学意义的下降（P = 0.04）。
结论
鉴于GT引起的NFκB和全身氧化变化以及GT多酚在前列腺组织中的摄取，未来有必要进行更长期的研究，以进一步研究GT在前列腺癌预防和治疗中的作用，以及可能在其他前列腺疾病如前列腺炎中的作用。
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