O'Geen Henriette, Yu Abigail S, Segal David J
Genome Center, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA 95616, USA.
Genome Center, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA 95616, USA.
Curr Opin Chem Biol. 2015 Dec;29:72-8. doi: 10.1016/j.cbpa.2015.10.001. Epub 2015 Oct 24.
The specificity of RNA-guided nucleases has gathered considerable interest as they become broadly applied to basic research and therapeutic development. Reports of the simple generation of animal models and genome engineering of cells raised questions about targeting precision. Conflicting early reports led the field to believe that CRISPR/Cas9 system was promiscuous, leading to a variety of strategies for improving specificity and increasingly sensitive methods to detect off-target events. However, other studies have suggested that CRISPR/Cas9 is a highly specific genome-editing tool. This review will focus on deciphering and interpreting these seemingly opposing claims.
随着RNA引导的核酸酶被广泛应用于基础研究和治疗开发，其特异性引起了人们的极大兴趣。关于简单生成动物模型和细胞基因组工程的报道引发了对靶向精度的质疑。早期相互矛盾的报道使该领域认为CRISPR/Cas9系统具有脱靶性，从而催生了各种提高特异性的策略以及检测脱靶事件的更灵敏方法。然而，其他研究表明CRISPR/Cas9是一种高度特异性的基因组编辑工具。本综述将聚焦于解读和阐释这些看似相互矛盾的观点。
