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酿酒酵母CNCM I-745菌株对炭疽杆菌致死毒素具有保护作用。

The Saccharomyces boulardii CNCM I-745 strain shows protective effects against the B. anthracis LT toxin.

作者信息

Pontier-Bres Rodolphe, Rampal Patrick, Peyron Jean-François, Munro Patrick, Lemichez Emmanuel, Czerucka Dorota

机构信息

Centre Scientifique de Monaco, Monaco 98000, Monaco.

Team Inflammation, Cancer, Cancer Stem Cells, Centre Méditerranéen de Médecine Moléculaire (C3M), INSERM, U1065, Nice 06204, France.

出版信息

Toxins (Basel). 2015 Oct 30;7(11):4455-67. doi: 10.3390/toxins7114455.

DOI:10.3390/toxins7114455
PMID:26529015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4663514/
Abstract

The probiotic yeast Saccharomyces boulardii (S. boulardii) has been prescribed for the prophylaxis and treatment of several infectious diarrheal diseases. Gastrointestinal anthrax causes fatal systemic disease. In the present study, we investigated the protective effects conferred by Saccharomyces boulardii CNCM I-745 strain on polarized T84 columnar epithelial cells intoxicated by the lethal toxin (LT) of Bacillus anthracis. Exposure of polarized T84 cells to LT affected cell monolayer integrity, modified the morphology of tight junctions and induced the formation of actin stress fibers. Overnight treatment of cells with S. boulardii before incubation with LT maintained the integrity of the monolayers, prevented morphological modification of tight junctions, restricted the effects of LT on actin remodeling and delayed LT-induced MEK-2 cleavage. Mechanistically, we demonstrated that in the presence of S. boulardii, the medium is depleted of both LF and PA sub-units of LT and the appearance of a cleaved form of PA. Our study highlights the potential of the S. boulardii CNCM I-745 strain as a prophylactic agent against the gastrointestinal form of anthrax.

摘要

益生菌酵母布拉氏酵母菌（S. boulardii）已被用于多种感染性腹泻疾病的预防和治疗。胃肠道炭疽会引发致命的全身性疾病。在本研究中，我们调查了布拉氏酵母菌CNCM I - 745菌株对被炭疽芽孢杆菌致死毒素（LT）毒害的极化T84柱状上皮细胞的保护作用。将极化的T84细胞暴露于LT会影响细胞单层的完整性，改变紧密连接的形态，并诱导肌动蛋白应激纤维的形成。在用LT孵育之前，用布拉氏酵母菌对细胞进行过夜处理可维持单层的完整性，防止紧密连接的形态改变，限制LT对肌动蛋白重塑的影响，并延迟LT诱导的MEK - 2裂解。从机制上讲，我们证明在有布拉氏酵母菌存在的情况下，培养基中LT的LF和PA亚基均被耗尽，并且出现了PA的裂解形式。我们的研究突出了布拉氏酵母菌CNCM I - 745菌株作为预防胃肠道型炭疽的潜在药物的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/49613e231eed/toxins-07-04455-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/c1fbcc9739db/toxins-07-04455-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/ccbb8599a25e/toxins-07-04455-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/f10aa0cd5d16/toxins-07-04455-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/89baf9bb9442/toxins-07-04455-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/49613e231eed/toxins-07-04455-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/c1fbcc9739db/toxins-07-04455-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/ccbb8599a25e/toxins-07-04455-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/f10aa0cd5d16/toxins-07-04455-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/89baf9bb9442/toxins-07-04455-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49e/4663514/49613e231eed/toxins-07-04455-g005.jpg

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Toxins (Basel). 2015 Sep 29;7(10):3960-76. doi: 10.3390/toxins7103960.
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Contractile actin cables induced by Bacillus anthracis lethal toxin depend on the histone acetylation machinery.炭疽芽孢杆菌致死毒素诱导产生的收缩性肌动蛋白束依赖于组蛋白乙酰化机制。
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Micropatterned macrophage analysis reveals global cytoskeleton constraints induced by Bacillus anthracis edema toxin.
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Saccharomyces cerevisiae Boulardii Reduces the Deoxynivalenol-Induced Alteration of the Intestinal Transcriptome.布拉氏酵母菌降低脱氧雪腐镰刀菌烯醇诱导的肠道转录组改变。
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