Reimer L G, Reller L B, Mirrett S
Microbiology Laboratory, Veterans Administration Medical Center, Salt Lake City, Utah.
J Clin Microbiol. 1989 Dec;27(12):2637-9. doi: 10.1128/jcm.27.12.2637-2639.1989.
Two commercially available agar slide blood culture systems, Septi-Chek (Roche Diagnostics, Div. Hoffmann-La Roche Inc., Nutley, N.J.) and VACUTAINER agar slant (Becton Dickinson [BD] Vacutainer Systems, Rutherford, N.J.), were compared in 5,628 paired blood cultures from adult patients. The systems were inoculated with equal volumes (5 ml) of blood. There was no statistically significant difference between the two systems in the overall recovery of clinically important microorganisms; however, significantly more Escherichia coli (P less than 0.05) organisms were detected in the Septi-Chek system. Concurrently, significantly more contaminants (P less than 0.001) also were present in Septi-Chek bottles. When microorganisms grew in both systems, there was no significant difference in the time to recovery of important organisms, except that anaerobes were detected more quickly in the BD system. This study suggests that the BD VACUTAINER agar slant is an acceptable alternative to the Septi-Chek system for routine blood cultures.
对两种市售的琼脂玻片血培养系统，即Septi-Chek（罗氏诊断公司，霍夫曼-罗氏有限公司分部，新泽西州纳特利）和VACUTAINER琼脂斜面（贝克顿·迪金森公司[BD]VACUTAINER系统，新泽西州卢瑟福），在来自成年患者的5628对血培养中进行了比较。这两种系统均接种等量（5毫升）血液。在临床上重要微生物的总体回收率方面，两种系统之间无统计学显著差异；然而，在Septi-Chek系统中检测到的大肠杆菌（P<0.05）明显更多。同时，Septi-Chek瓶中的污染物（P<0.001）也明显更多。当两种系统中均有微生物生长时，重要微生物的恢复时间无显著差异，只是在BD系统中厌氧菌的检测更快。本研究表明，对于常规血培养，BD VACUTAINER琼脂斜面是Septi-Chek系统的可接受替代方案。
