Bao Peng, Xiao Ke-Qing, Wang Hui-Jiao, Xu Hao, Xu Peng-Peng, Jia Yan, Häggblom Max M, Zhu Yong-Guan
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, P. R. China.
Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China.
Sci Rep. 2016 Sep 28;6:34054. doi: 10.1038/srep34054.
A novel nitrate- and selenite reducing bacterium strain ZYK was isolated from a rice paddy soil in Dehong, Yunnan, China. Strain ZYK is a facultative anaerobe and grows in up to 150, 000 ppm O. The comparative genomics analysis of strain ZYK implies that it shares more orthologues with B. subtilis subsp. subtilis NCIB 3610 (ANIm values, 85.4-86.7%) than with B. azotoformans NBRC 15712 (ANIm values, 84.4-84.7%), although B. azotoformans NBRC 15712 (96.3% 16S rRNA gene sequence similarity) is the closest Bacillus species according to 16S rRNA gene comparison. The major cellular fatty acids of strain ZYK were iso-C (17.8%), iso-C (17.8%), and C (32.0%). The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. Based on physiological, biochemical and genotypic properties, the strain was considered to represent a novel species of the genus Bacillus, for which the name Bacillus oryziterrae sp. nov. is proposed. The type strain is ZYK (=DSM 26460 =CGMCC 1.5179). Strain ZYK can reduce nitrate to nitrite and ammonium and possesses metabolic genes for nitrate reduction including nar, nap and nrf. Biogenic selenium nanoparticles of strain ZYK show a narrow size distribution and agree with the gaussian distribution. These selenium nanoparticles show significant dose-dependent inhibition of the lung cancer cell line H157, which suggests potential for application in cancer therapy.
从中国云南德宏的稻田土壤中分离出一株新型的硝酸盐和亚硒酸盐还原细菌菌株ZYK。菌株ZYK是兼性厌氧菌，能在高达150,000 ppm的氧气中生长。对菌株ZYK的比较基因组学分析表明，与氮芽孢杆菌NBRC 15712（ANI值为84.4-84.7%）相比，它与枯草芽孢杆菌枯草亚种NCIB 3610共享更多的直系同源基因（ANI值为85.4-86.7%），尽管根据16S rRNA基因比较，氮芽孢杆菌NBRC 15712（16S rRNA基因序列相似性为96.3%）是最接近的芽孢杆菌属物种。菌株ZYK的主要细胞脂肪酸为异-C（17.8%）、异-C（17.8%）和C（32.0%）。极性脂质谱由磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油和一种未鉴定的氨基磷脂组成。基于生理、生化和基因型特性，该菌株被认为代表芽孢杆菌属的一个新物种，为此提出了芽孢杆菌稻土新种的名称。模式菌株为ZYK（=DSM 26460 =CGMCC 1.5179）。菌株ZYK能将硝酸盐还原为亚硝酸盐和铵，并拥有包括nar、nap和nrf在内的硝酸盐还原代谢基因。菌株ZYK产生的生物源硒纳米颗粒尺寸分布狭窄，符合高斯分布。这些硒纳米颗粒对肺癌细胞系H157表现出显著的剂量依赖性抑制作用，这表明其在癌症治疗中具有应用潜力。
