Brinkmann Christian, Metten Alexander, Scriba Philipp, Tagarakis Christos V M, Wahl Patrick, Latsch Jo, Brixius Klara, Bloch Wilhelm
Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany.
Institute of Training Science and Sport Informatics, German Sport University Cologne, Cologne, Germany.
Int J Sports Med. 2017 Feb;38(2):92-98. doi: 10.1055/s-0042-116823. Epub 2017 Jan 9.
Exercise-induced transient increases in pro-angiogenic regulators can promote angiogenesis.This pilot study aims to analyze the potential of exercise to positively affect angiogenic regulators in patients with type 2 diabetes mellitus (T2DM), who often exhibit abnormal angiogenesis, under different environmental conditions. 9 overweight/obese men with uncomplicated T2DM (8 took anti-diabetic drugs) performed submaximal cycling for 40 min in normoxia (≈21 vol%O), hypoxia (≈14 vol%O) and during alternating hypoxia/hyperoxia (≈14 vol%O/≈30 vol%O, 5-min intervals) (3×3 crossover design). Serum pro-angiogenic vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-9 and anti-angiogenic endostatin were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Non-parametric statistical tests (Wilcoxon, Friedman analysis of variance) were applied. VEGF increased significantly from pre- to post-exercise with hypoxia and hypoxia/hyperoxia. MMP-2 increased significantly in all experimental runs, while MMP-9 only increased significantly with hypoxia and hypoxia/hyperoxia. Endostatin increased significantly with normoxia and hypoxia. However, the magnitude of changes did not differ significantly between conditions. Capillary blood lactate was significantly lower following cycling with hypoxia/hyperoxia than with hypoxia alone. Although differences in subjective ratings of perceived exertion failed significance, 7 subjects were less exerted with hypoxia/hyperoxia than with hypoxia. Submaximal cycling with hypoxia or alternating hypoxia/hyperoxia may induce a more reliable up-regulation of pro-angiogenic regulators compared with normoxia, while hypoxia/hyperoxia may be better tolerated than hypoxia alone.
运动诱导的促血管生成调节因子的短暂增加可促进血管生成。这项初步研究旨在分析在不同环境条件下，运动对2型糖尿病（T2DM）患者血管生成调节因子产生积极影响的潜力，T2DM患者常表现出异常的血管生成。9名患有非复杂性T2DM的超重/肥胖男性（8人服用抗糖尿病药物）在常氧（≈21%体积的氧气）、低氧（≈14%体积的氧气）以及低氧/高氧交替（≈14%体积的氧气/≈30%体积的氧气，5分钟间隔）条件下进行了40分钟的次最大强度骑行（3×3交叉设计）。使用酶联免疫吸附测定（ELISA）试剂盒对血清促血管生成的血管内皮生长因子（VEGF）、基质金属蛋白酶（MMP）-2、MMP-9和抗血管生成的内皮抑素进行定量分析。应用非参数统计检验（威尔科克森检验、弗里德曼方差分析）。在低氧和低氧/高氧条件下，运动后VEGF较运动前显著增加。在所有实验过程中MMP-2均显著增加，而MMP-9仅在低氧和低氧/高氧条件下显著增加。内皮抑素在常氧和低氧条件下显著增加。然而，不同条件下变化的幅度没有显著差异。与单纯低氧骑行相比，低氧/高氧交替骑行后毛细血管血乳酸显著降低。尽管主观用力感觉评分的差异不显著，但7名受试者在低氧/高氧条件下比在低氧条件下感觉用力程度更低。与常氧相比，低氧或低氧/高氧交替的次最大强度骑行可能会诱导促血管生成调节因子更可靠的上调，而低氧/高氧可能比单纯低氧耐受性更好。
