Alving B M, Banerji B, Fogler W E, Alving C R
Department of Hematology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Clin Exp Immunol. 1987 Aug;69(2):403-8.
Four murine monoclonal antibodies having high levels of activity against phosphatidyl-inositol phosphate (PIP) were tested for lupus anticoagulant activity. The antibodies showed different degrees of potency in a modified partial thromboplastin time test (APTT) that used dilutions of either bovine brain extract (Thrombofax) or liposomes consisting of phosphatidylcholine/phosphatidylserine (PC/PS) as the phospholipid source. The same relative order of anticoagulant potency that was observed in the APTT that used the PC/PS liposomes was maintained when the anti-PIP antibodies were tested for cross-reactivity either by induction of complement-dependent immune damage to liposomes containing PS, or in enzyme-linked immunosorbent assays that used PS, cardiolipin (CL), or phosphatidylinositol (PI) as antigens. The data indicate that monoclonal antibodies to PIP can express anticoagulant activity in a modified APTT that correlates with their different degrees of cross-reactivity against the negatively-charged phospholipids PS, CL, and PI.
对四种针对磷脂酰肌醇磷酸（PIP）具有高活性的鼠单克隆抗体进行狼疮抗凝活性测试。在改良的部分凝血活酶时间试验（APTT）中，这些抗体表现出不同程度的效力，该试验使用牛脑提取物（凝血因子）或由磷脂酰胆碱/磷脂酰丝氨酸（PC/PS）组成的脂质体作为磷脂来源的稀释液。当通过对含有PS的脂质体进行补体依赖性免疫损伤诱导，或在使用PS、心磷脂（CL）或磷脂酰肌醇（PI）作为抗原的酶联免疫吸附试验中测试抗PIP抗体的交叉反应性时，在使用PC/PS脂质体的APTT中观察到的相同抗凝效力相对顺序得以维持。数据表明，针对PIP的单克隆抗体可以在改良的APTT中表达抗凝活性，这与其对带负电荷的磷脂PS、CL和PI的不同程度交叉反应性相关。
