Li Yong, Xue Han, Sang Sheng-Qi, Lin Cai-Li, Wang Xi-Zhuo
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing, China.
Agricultural High and New Technology Development Center of Puyang, Puyang, China.
PLoS One. 2017 Apr 13;12(4):e0174506. doi: 10.1371/journal.pone.0174506. eCollection 2017.
Two Gram-stain negative aerobic bacterial strains were isolated from the bark tissue of Populus × euramericana. The novel isolates were investigated using a polyphasic approach including 16S rRNA gene sequencing, genome sequencing, average nucleotide identity (ANI) and both phenotypic and chemotaxonomic assays. The genome core gene sequence and 16S rRNA gene phylogenies suggest that the novel isolates are different from the genera Snodgrassella and Stenoxybacter. Additionally, the ANI, G+C content, main fatty acids and phospholipid profile data supported the distinctiveness of the novel strain from genus Snodgrassella. Therefore, based on the data presented, the strains constitute a novel species of a novel genus within the family Neisseriaceae, for which the name Populibacter corticis gen. nov., sp. nov. is proposed. The type strain is 15-3-5T (= CFCC 13594T = KCTC 42251T).
从欧美杨的树皮组织中分离出两株革兰氏染色阴性需氧细菌菌株。采用多相分类方法对新分离菌株进行了研究，包括16S rRNA基因测序、基因组测序、平均核苷酸同一性（ANI）以及表型和化学分类分析。基因组核心基因序列和16S rRNA基因系统发育分析表明，新分离菌株与斯诺德氏菌属和窄杆菌属不同。此外，ANI、G+C含量、主要脂肪酸和磷脂谱数据支持新菌株与斯诺德氏菌属的独特性。因此，根据所提供的数据，这些菌株构成了奈瑟菌科一个新属的一个新物种，为此提出了新属名Populibacter corticis gen. nov.，新种名Populibacter corticis sp. nov.。模式菌株为15-3-5T（=CFCC 13594T=KCTC 42251T）。
