Ben Hmad Ines, Gargouri Ali
Laboratory of Molecular Biology of Eukaryotes, Centre of Biotechnology of Sfax CBS/University of Sfax, Sfax, Tunisia.
J Basic Microbiol. 2017 Aug;57(8):653-658. doi: 10.1002/jobm.201700111. Epub 2017 May 15.
Neutral and alkaline cellulases from microorganisms constitute a major group of the industrial enzymes and find applications in various industries. Screening is the important ways to get novel cellulases. Most fungal cellulases have acidic pH optima, except some fungi like Humicola insolens species. However, new applications require the use of neutral and alkaline cellulases in food, brewery and wine, animal feed, textile and laundry, pulp and paper industries, agriculture as well in scientific research purposes. Indeed, the demand for these enzymes is growing more rapidly than ever before, and becomes the driving force for research on engineering the cellulolytic enzymes. Here, we present an overview of the biotechnological research for neutral and alkaline cellulases.
来自微生物的中性和碱性纤维素酶构成了工业酶的主要类别，并在各个行业中得到应用。筛选是获得新型纤维素酶的重要途径。除了像特异腐质霉等一些真菌外，大多数真菌纤维素酶的最适pH值呈酸性。然而，食品、啤酒和葡萄酒、动物饲料、纺织和洗衣、制浆造纸行业、农业以及科学研究目的等新应用需要使用中性和碱性纤维素酶。事实上，对这些酶的需求正以前所未有的速度增长，并成为纤维素分解酶工程研究的驱动力。在此，我们概述了中性和碱性纤维素酶的生物技术研究。
