Ahangarpour Akram, Oroojan Ali Akbar, Khorsandi Layasadat, Najimi Seyedeh Asma
Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R. Iran.
Department of Physiology, Student Research Committee of Ahvaz Jundishapur University of Medical Science, Ahvaz, I.R. Iran.
Res Pharm Sci. 2017 Apr;12(2):137-143. doi: 10.4103/1735-5362.202452.
D-galactose induces pancreatic disorder along with aging mouse model. (VAC) has potential pancreatic protective effect. Hence, this study was designed to evaluate the hypoglycemic and pancreas protective effects of VAC hydroalcoholic extract in D-galactose-induced aging female mice. In the present experimental study, 72 adult female Naval Medical Research Institute (NMRI) mice (weighing 30-35 g) were divided into 6 groups of control, VAC hydroalcoholic extract, D-galactose, D-galactose + VAC hydroalcoholic extract, aged, aged + VAC hydroalcoholic extract. The aged model was prepared by subcutaneous injection of D-galactose for 45 days and, VAC hydroalcoholic extract was gavaged twice a day in the last 7 days. 24 h after the last drug and extract administrations, serum samples and pancreatic tissues were removed to evaluate experimental and histological determinations. Serum glucose level decreased in VAC, D-galactose and, aged-treated groups compared to the control ( < 0.05). Insulin level increased in VAC and decreased in D-galactose and aged VAC-treated mice compared to the control ( < 0.05). Homeostasis model assessment-estimated insulin resistance (HOMA-IR) increased in D-galactose, aging, and VAC hydroalcoholic extract groups ( < 0.05) and, administration of VAC hydroalcoholic extract improved HOMA-IR in D-galactose and aging treated animals. Despite the size of pancreatic islets decreased in aged and D-galactose groups, VAC administration recovered it. Present data showed that VAC hydroalcoholic extract has hypoglycemic and pancreatic protective effects in natural aged and aging model mice.
D-半乳糖与衰老小鼠模型一起会诱发胰腺紊乱。（VAC）具有潜在的胰腺保护作用。因此，本研究旨在评估VAC水醇提取物对D-半乳糖诱导的衰老雌性小鼠的降血糖和胰腺保护作用。在本实验研究中，72只成年雌性海军医学研究所（NMRI）小鼠（体重30 - 35克）被分为6组，即对照组、VAC水醇提取物组、D-半乳糖组、D-半乳糖 + VAC水醇提取物组、老龄组、老龄 + VAC水醇提取物组。通过皮下注射D-半乳糖45天制备衰老模型，在最后7天每天两次灌胃给予VAC水醇提取物。在最后一次给药和提取物处理24小时后，采集血清样本和胰腺组织以评估实验和组织学测定。与对照组相比，VAC组、D-半乳糖组和老龄处理组的血清葡萄糖水平降低（<0.05）。与对照组相比，VAC组胰岛素水平升高，D-半乳糖组和老龄VAC处理组胰岛素水平降低（<0.05）。D-半乳糖组、老龄组和VAC水醇提取物组的稳态模型评估 - 估计胰岛素抵抗（HOMA-IR）升高（<0.05），给予VAC水醇提取物可改善D-半乳糖组和老龄处理动物的HOMA-IR。尽管老龄组和D-半乳糖组的胰岛大小减小，但给予VAC可使其恢复。目前的数据表明，VAC水醇提取物对自然衰老和衰老模型小鼠具有降血糖和胰腺保护作用。
