Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Santiago 7820436, Chile.
Int J Mol Sci. 2017 Oct 4;18(10):2095. doi: 10.3390/ijms18102095.
Composition of the gut microbiome is influenced by diet. Milk or formula oligosaccharides act as prebiotics, bioactives that promote the growth of beneficial gut microbes. The influence of prebiotics on microbial interactions is not well understood. Here we investigated the transformation of prebiotics by a consortium of four representative species of the infant gut microbiome, and how their interactions changed with dietary substrates. First, we optimized a culture medium resembling certain infant gut parameters. A consortium containing subsp. , , and was grown on fructooligosaccharides (FOS) or 2'-fucosyllactose (2FL) in mono- or co-culture. While and dominated growth on 2FL, their combined growth was reduced. Besides, interaction coefficients indicated strong competition, especially on FOS. While FOS was rapidly consumed by the consortium, was the only microbe displaying significant consumption of 2FL. Acid production by the consortium resembled the metabolism of microorganisms dominating growth in each substrate. Finally, the consortium was tested in a bioreactor, observing similar predominance but more pronounced acid production and substrate consumption. This study indicates that the chemical nature of prebiotics modulate microbial interactions in a consortium of infant gut species.
肠道微生物组的组成受饮食影响。牛奶或配方中的低聚糖作为益生元，可促进有益肠道微生物的生长。益生元对微生物相互作用的影响尚不清楚。在这里，我们研究了婴儿肠道微生物组的四种代表性物种的混合物对益生元的转化，以及它们的相互作用如何随饮食底物而变化。首先，我们优化了一种类似于某些婴儿肠道参数的培养基。含有 亚种、、和 的混合物在单培养或共培养中以果寡糖（FOS）或 2'-岩藻糖基乳糖（2FL）为底物进行生长。虽然 和 优先在 2FL 上生长，但它们的共同生长受到抑制。此外，相互作用系数表明存在强烈的竞争，尤其是在 FOS 上。虽然 FOS 被混合物迅速消耗，但只有 是唯一显示出对 2FL 有显著消耗的微生物。混合物的产酸作用类似于在每种底物中占主导地位的微生物的代谢。最后，我们在生物反应器中测试了该混合物，观察到类似的优势，但产酸和底物消耗更为明显。本研究表明，益生元的化学性质调节了婴儿肠道物种混合物中的微生物相互作用。
