College of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai'an 271018, China.
Molecules. 2017 Nov 7;22(11):1918. doi: 10.3390/molecules22111918.
The study is aimed at investigating the immunoenhancement activity of polysaccharides from polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4⁺ and CD8⁺ T cells were performed. Four different concentrations of PGPSs (PGPS, PGPS, PGPS, and PGPS) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPS groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPS and PGPS were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4⁺ and CD8⁺ T cells. At most time points, PGPS could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4⁺ and CD8⁺ T cells. These results indicate that PGPS enhances the immune functions, suggesting that PGPS could be a potential immunopotentiator for further in vivo and clinical trial experiments.
本研究旨在体外研究多糖（PGPSs）的多糖的免疫增强活性。在这项研究中，我们对淋巴细胞增殖、细胞周期以及 CD4⁺和 CD8⁺T 细胞水平进行了一些研究。我们收获了四种不同浓度的 PGPS（PGPS、PGPS、PGPS 和 PGPS），并将其添加到外周血 T 淋巴细胞中。我们观察到，在大多数浓度下，PGPS 组或与植物血凝素（PHA）协同作用均可显著增加 T 淋巴细胞增殖，且其淋巴细胞增殖率最高。随后选择 PGPS 和 PGPS 的活性部位。然后，我们利用流式细胞术来确定淋巴细胞细胞周期分布和 CD4⁺和 CD8⁺T 细胞的水平。在大多数时间点，PGPS 可以促进淋巴细胞从 G0/G1 期向 S 和 G2/M 期的细胞周期进程，并同时增加 CD4⁺和 CD8⁺T 细胞的水平。这些结果表明 PGPS 增强了免疫功能，提示 PGPS 可能是进一步进行体内和临床试验的潜在免疫增强剂。
