Belova Svetlana E, Suzina Natalia E, Rijpstra W Irene C, Sinninghe Damsté Jaap S, Dedysh Svetlana N
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia.
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino, Russia.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1265-1270. doi: 10.1099/ijsem.0.002663.
An isolate of aerobic, Gram-stain-negative, rod-shaped, non-motile and light-pink pigmented bacteria, designated SBC68, was obtained from slightly decomposed thalli of the lichen sp. collected from the forested tundra of north-western Siberia. Cells of this isolate occurred singly, in pairs or in rosettes. These bacteria were acidophilic (optimum growth at pH 4.3-5.6) and mesophilic (optimum growth at 20-30 °C) but were also capable of growth at low temperatures, down to 7 °C. The preferred growth substrates were sugars, some organic acids and lichenan. The major fatty acids were iso-C, Cω7, C, Cω7, and 13,16-dimethyl octacosanedioic acid. The only quinone was MK-8, and the G+C content of the DNA was 54.7 mol%. SBC68 represented a member of the family ; the closest taxonomically described relatives were DHF9 and TPB6028 (97.2 and 97.1 % 16S rRNA gene sequence similarity, respectively). In 16S rRNA gene-based trees, SBC68 clustered together with species of the genus . However, this isolate differed from all previously described species of the genus with respect to the pink pigmentation, formation of cell rosettes and substrate utilization pattern. On the basis of these data, strain SBC68 should be considered to represent a novel species of acidobacteria, for which the name sp. nov. is proposed. The type strain is SBC68 (=DSM 104462=VKM B-3208).
从西伯利亚西北部森林苔原采集的地衣属植物略有腐烂的叶状体中，分离出一株需氧、革兰氏染色阴性、杆状、无运动性且呈浅粉色色素的细菌，命名为SBC68。该分离株的细胞单个、成对或呈玫瑰花结状出现。这些细菌嗜酸（最适生长pH为4.3 - 5.6）且嗜温（最适生长温度为20 - 30°C），但也能在低温下生长，低至7°C。优选的生长底物是糖类、一些有机酸和地衣聚糖。主要脂肪酸为异-C、Cω7、C、Cω7和13,16 - 二甲基二十八烷二酸。唯一的醌是MK - 8，DNA的G + C含量为54.7 mol%。SBC68代表该科的一个成员；分类学上描述的最接近的亲缘种是DHF9和TPB6028（16S rRNA基因序列相似性分别为97.2%和97.1%）。在基于16S rRNA基因的系统发育树中，SBC68与该属的物种聚在一起。然而，该分离株在粉色色素沉着、细胞玫瑰花结形成和底物利用模式方面与该属所有先前描述的物种不同。基于这些数据，菌株SBC68应被视为代表酸杆菌属的一个新物种，为此提出新名称酸杆菌属新种。模式菌株是SBC68（=DSM 104462 =VKM B - 3208）。
