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体外实验中利用 PLGA/氧化铁微颗粒对肌腱干细胞进行双模式光声和磁共振追踪。

Dual-modal photoacoustic and magnetic resonance tracking of tendon stem cells with PLGA/iron oxide microparticles in vitro.

机构信息

Chongqing Key laboratory of Ultrasound Molecular Imaging, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Ultrasound Medical Center, Sichuan Cancer Hospital Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

出版信息

PLoS One. 2018 Apr 2;13(4):e0193362. doi: 10.1371/journal.pone.0193362. eCollection 2018.

DOI:10.1371/journal.pone.0193362
PMID:29608568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5880337/
Abstract

Reliable cell tracking is essential to understand the fate of stem cells following implantation, and thus promote the clinical application of stem cell therapy. Dual or multiple modal imaging modalities mediated by different types of multifunctional contrast agent are generally needed for efficient cell tracking. Here, we created a new contrast agent-PLGA/iron oxide microparticles (PLGA/IO MPs) and characterized the morphology, structure and function of enhancing both photoacoustic (PA) and magnetic resonance imaging (MRI). Both PA and MRI signal increased with increased Fe concentration of PLGA/IO MPs. Fluorescent staining, Prussian blue staining and transmission electron microscope (TEM) certified that PLGA/IO MPs were successfully encapsulated in the labeled TSCs. The established PLGA/IO MPs demonstrated superior ability of dual-modal PA/MRI tracking of TSCs without cytotoxicity at relatively lower Fe concentrations (50, 100 and 200 μg/mL). The optimal Fe concentration of PLGA/IO MPs was determined to be 100 μg/mL, thus laying a foundation for the further study of dual-modal PA/MRI tracking of TSCs in vivo and promoting the repair of injured tendon.

摘要

可靠的细胞示踪对于了解植入后干细胞的命运至关重要，从而促进干细胞治疗的临床应用。通常需要通过不同类型的多功能造影剂介导的双模态或多模态成像方式来进行有效的细胞示踪。在这里，我们制备了一种新型造影剂——聚乳酸-羟基乙酸共聚物/氧化铁微粒（PLGA/IO MPs），并对其增强光声（PA）和磁共振成像（MRI）的形态、结构和功能进行了表征。PA 和 MRI 信号均随 PLGA/IO MPs 中 Fe 浓度的增加而增强。荧光染色、普鲁士蓝染色和透射电子显微镜（TEM）证实 PLGA/IO MPs 已成功包被在标记的 TSCs 中。所建立的 PLGA/IO MPs 在相对较低的 Fe 浓度（50、100 和 200μg/mL）下表现出优异的 TSCs 双模态 PA/MRI 示踪能力，且无细胞毒性。PLGA/IO MPs 的最佳 Fe 浓度确定为 100μg/mL，从而为进一步研究 TSCs 的体内双模态 PA/MRI 示踪奠定了基础，并促进了受损肌腱的修复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/ac89813f738b/pone.0193362.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/de1b6e32baac/pone.0193362.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/541d51fe37e6/pone.0193362.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/2a6d1f9c69e8/pone.0193362.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/ac89813f738b/pone.0193362.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/221773af1d20/pone.0193362.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/300a333599cc/pone.0193362.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/7fe85acce5cd/pone.0193362.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/de1b6e32baac/pone.0193362.g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/2a6d1f9c69e8/pone.0193362.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e28/5880337/ac89813f738b/pone.0193362.g007.jpg

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