Sabarneh Anas, Ereqat Suheir, Cauchi Stéphane, AbuShamma Omar, Abdelhafez Mohammad, Ibrahim Murad, Nasereddin Abdelmajeed
Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Dis-East Jerusalem, Palestine.
Al-Quds Nutrition and Health Research Institute - Faculty of Medicine, Al-Quds University-Palestine, Abu Dis-Jerusalem, Palestine.
BMC Med Genet. 2018 Aug 31;19(1):156. doi: 10.1186/s12881-018-0668-8.
Genetic and environmental factors play a crucial role in the development of type 2 diabetes mellitus (T2DM) and obesity. This study aimed to investigate the association of the fat-mass and obesity-associated gene (FTO) rs9939609 variant with T2DM and body mass index (BMI) among Palestinian population.
A total of 399 subjects were recruited, of whom 281 were type 2 diabetic patients and 118 normoglycemic subjects. All of them were unrelated, aged > 40 years and recruited within the period 2016-2017. The A allele of FTO rs9939609 was identified by PCR-RFLP.
Significant association of the minor allele A of FTO rs9939609 and T2DM risk was observed with an allelic odd ratio of 1.92 (95% CI [1.09-3.29], p = 0.02) adjusted for age and gender, this association partly attenuated when adjusted for BMI with OR of 1.84, (95%CI [1.04-3.05], p = 0.03). Stratified data by glycemic status across FTO genotypes showed that A allele was marginally associated with increased BMI among diabetic group (p = 0.057) but not in control group (p = 0.7). Moreover, no significant association was observed between FTO genotypes and covariates of age, gender, T2DM complications or any tested metabolic trait in both diabetic and nondiabetic individuals (p > 0.05).
The variant rs9939609 of the FTO gene was associated with T2DM in Palestine. This is the first study conducted on this gene in the Palestinian population and provides valuable information for comparison with other ethnic groups. Further analysis with larger sample size is required to elucidate the role of this variant on the predisposition to increased BMI in Palestinians.
遗传和环境因素在2型糖尿病（T2DM）和肥胖症的发生发展中起着至关重要的作用。本研究旨在调查巴勒斯坦人群中脂肪量和肥胖相关基因（FTO）rs9939609变异与T2DM及体重指数（BMI）之间的关联。
共招募了399名受试者，其中281名是2型糖尿病患者，118名是血糖正常的受试者。他们均无亲缘关系，年龄大于40岁，于2016 - 2017年期间招募。通过聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）鉴定FTO rs9939609的A等位基因。
观察到FTO rs9939609的次要等位基因A与T2DM风险存在显著关联，在调整年龄和性别后，等位基因比值比为1.92（95%置信区间[1.09 - 3.29]，p = 0.02）；在调整BMI后，这种关联部分减弱，比值比为1.84（95%置信区间[1.04 - 3.05]，p = 0.03）。按血糖状态对FTO基因型进行分层的数据显示，A等位基因在糖尿病组中与BMI升高存在边缘关联（p = 0.057），而在对照组中无此关联（p = 0.7）。此外，在糖尿病和非糖尿病个体中，未观察到FTO基因型与年龄、性别、T2DM并发症或任何检测的代谢特征的协变量之间存在显著关联（p > 0.05）。
FTO基因的rs9939609变异与巴勒斯坦人群的T2DM相关。这是在巴勒斯坦人群中对该基因进行的首次研究，为与其他种族群体进行比较提供了有价值的信息。需要更大样本量的进一步分析来阐明该变异对巴勒斯坦人BMI升高易感性的作用。
