School of Pharmacy, China Medical University, Taichung, 404, Taiwan.
Chinese Medicine Research and Development Center and Department of Medical Research, China Medical University Hospital, Taichung, 404, Taiwan.
Sci Rep. 2018 Oct 18;8(1):15417. doi: 10.1038/s41598-018-33748-6.
Glaulactams A-C (1-3), which possess a novel skeleton, as well as the known compound daphmanidin B (4), were isolated from the leaves of Daphniphyllum glaucescens and separated using ion-exchange chromatography aided by NMR fingerprinting. Their structures, including their absolute configurations, were elucidated by spectroscopic analyses and time-dependent density-functional-theory-calculated electronic circular dichroism spectra; the data were subsequently analyzed to gain insight into the respective biogenetic relationships between the isolates, which exhibited anti-H1N1 and immunosuppressive activities.
从广西冷水花中分离得到具有新颖骨架的倍半萜 glaulactams A-C（1-3）以及已知化合物 daphniphyllumglaucescens B（4），采用离子交换色谱法结合 NMR 指纹图谱进行分离。通过光谱分析和时间依赖密度泛函理论计算的电子圆二色谱光谱解析了它们的结构，包括它们的绝对构型；随后对数据进行了分析，以深入了解这些分离物之间的各自生物合成关系，这些分离物具有抗 H1N1 和免疫抑制活性。
