Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, Suruga-ku, Shizuoka Japan.
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, Suruga-ku, Shizuoka Japan.
PLoS One. 2019 Jan 31;14(1):e0211517. doi: 10.1371/journal.pone.0211517. eCollection 2019.
Among the members of flaviviruses, the Zika virus (ZIKV) remains a potent infectious disease agent, with its associated pandemic prompting the World Health Organization (WHO) to declare it a global public health concern. Thus, rapid and accurate diagnosis of the ZIKV is needed. In this study, we report a new immunofluorescence biosensor for the detection of nonstructural protein 1 (NS1) of the ZIKV, which operates using the localized surface plasmon resonance (LSPR) signal from plasmonic gold nanoparticles (AuNPs) to amplify the fluorescence intensity signal of quantum dots (QDs) within an antigen-antibody detection process. The LSPR signal from the AuNPs was used to amplify the fluorescence intensity of the QDs. For ultrasensitive, rapid, and quantitative detection of NS1 of the ZIKV, four different thiol-capped AuNPs were investigated. Our biosensor could detect the ZIKV in a wide concentration range from 10-107 RNA copies/mL, and we found that the limit of detection (LOD) for the ZIKV followed the order Ab-L-cysteine-AuNPs (LOD = 8.2 copies/mL) > Ab-3-mercaptopropionic acid-AuNPs (LOD = 35.0 copies/mL). Immunofluorescence biosensor for NS1 exhibited excellent specificity against other negative control targets and could also detect the ZIKV in human serum.
在黄病毒属成员中，寨卡病毒（ZIKV）仍然是一种强有力的传染性疾病病原体，其相关的大流行促使世界卫生组织（WHO）宣布其为全球公共卫生关注问题。因此，需要快速准确地诊断 ZIKV。在这项研究中，我们报告了一种用于检测寨卡病毒非结构蛋白 1（NS1）的新型免疫荧光生物传感器，该传感器利用等离子体金纳米粒子（AuNPs）的局域表面等离子体共振（LSPR）信号来放大抗原-抗体检测过程中量子点（QDs）的荧光强度信号。AuNPs 的 LSPR 信号用于放大 QDs 的荧光强度。为了对 ZIKV 的 NS1 进行超灵敏、快速和定量检测，研究了四种不同的巯基封端的 AuNPs。我们的生物传感器可以在 10-107 RNA 拷贝/mL 的宽浓度范围内检测 ZIKV，并且我们发现 ZIKV 的检测限（LOD）遵循 Ab-L-半胱氨酸-AuNPs（LOD=8.2 拷贝/mL）>Ab-3-巯基丙酸-AuNPs（LOD=35.0 拷贝/mL）的顺序。针对其他阴性对照靶标，NS1 的免疫荧光生物传感器表现出优异的特异性，并且还可以检测人血清中的 ZIKV。
