CRISPR 引导的剪接体进化以抵抗剪接抑制剂。
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors.
机构信息
Laboratory for Genome Engineering and Synthetic Biology, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Division of Biological and Environmental Sciences and Engineering, Computational Bioscience Research Center, KAUST, Thuwal, Saudi Arabia.
出版信息
Genome Biol. 2019 Apr 30;20(1):73. doi: 10.1186/s13059-019-1680-9.
Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions.
通过定向进化增加遗传多样性，为加速性状的发展和作物改良提供了巨大的前景。我们在植物中开发了一个基于 CRISPR/Cas 的定向进化平台，用于进化水稻（Oryza sativa）SF3B1 剪接体蛋白，以提高对剪接抑制剂的抗性。SF3B1 突变体变体，称为 SF3B1-GEX1A-Resistant (SGR)，赋予对剪接抑制剂不同程度的抗性。对剪接抑制剂与 SGR 结合的结构基础的研究证实了这种抗性表型。这个定向进化平台可用于研究和进化关键生物分子的分子功能，并为提高作物在气候变化条件下的性能和适应性而设计作物特性。
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