Department of Clinical and Molecular Medicine, School of Medicine and Psychology, Sapienza University of Rome, Italy.
IRCCS Neuromed, Pozzilli (Isernia), Italy.
Oxid Med Cell Longev. 2019 Oct 15;2019:2650429. doi: 10.1155/2019/2650429. eCollection 2019.
Increased oxidative stress from both mitochondrial and cytosolic sources contributes to the development and the progression of cardiovascular diseases (CVDs), and it is a target of therapeutic interventions. The numerous efforts made over the last decades in order to develop tools able to monitor the oxidative stress level in patients affected by CVDs rely on the need to gain information on the disease state. However, this goal has not been satisfactorily accomplished until now. Among others, the isolation of circulating leukocytes to measure their oxidant level offers a valid, noninvasive challenge that has been tested in few pathological contexts, including hypertension, atherosclerosis and its clinical manifestations, and heart failure. Since leukocytes circulate in the blood stream, it is expected that they might reflect quite closely both systemic and cardiovascular oxidative stress and provide useful information on the pathological condition. The results of the studies discussed in the present review article are promising. They highlight the importance of measuring oxidative stress level in circulating mononuclear cells in different CVDs with a consistent correlation between degree of oxidative stress and severity of CVD and of its complications. Importantly, they also point to a double role of leukocytes, both as a marker of disease condition and as a direct contributor to disease progression. Finally, they show that the oxidative stress level of leukocytes reflects the impact of therapeutic interventions. It is likely that the isolation of leukocytes and the measurement of oxidative stress, once adequately developed, may represent an eligible tool for both research and clinical purposes to monitor the role of oxidative stress on the promotion and progression of CVDs, as well as the impact of therapies.
来自线粒体和细胞质来源的氧化应激增加导致心血管疾病 (CVDs) 的发展和进展，这也是治疗干预的目标。在过去几十年中，为了开发能够监测 CVD 患者氧化应激水平的工具，人们做出了许多努力，这是为了满足获得有关疾病状态信息的需求。然而，到目前为止，这一目标还没有得到令人满意的实现。其中，分离循环白细胞以测量其氧化剂水平提供了一种有效的、非侵入性的挑战，这种方法已经在少数病理情况下进行了测试，包括高血压、动脉粥样硬化及其临床表现和心力衰竭。由于白细胞在血流中循环，预计它们可以非常密切地反映全身和心血管的氧化应激，并提供有关病理状况的有用信息。本文综述中讨论的研究结果是有希望的。它们强调了在不同 CVD 中测量循环单核细胞中氧化应激水平的重要性，氧化应激程度与 CVD 及其并发症的严重程度之间存在一致的相关性。重要的是，它们还指出白细胞具有双重作用，既是疾病状况的标志物，又是疾病进展的直接原因。最后，它们表明白细胞的氧化应激水平反映了治疗干预的影响。白细胞的分离和氧化应激的测量一旦得到充分发展，很可能成为研究和临床目的的一种合适工具，以监测氧化应激在促进和进展 CVD 以及治疗影响方面的作用。
