Federal Research Centre of Nutrition and Biotechnology, Ust'inskiy pr., 2/14, 109240 Moscow, Russia.
Toxins (Basel). 2020 Jan 30;12(2):94. doi: 10.3390/toxins12020094.
Standard solutions of mycotoxins prepared in RP HPLC solvents from neat standards are usually used for analytical method development. Multi-mycotoxin HPLC-MS/MS methods necessitate stability estimation for the wide spectrum of fungal metabolites. The stability of individual diluted stock standard solutions of mycotoxins in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants was evaluated under standard storage and analysis conditions. Individual stock standard solutions of aflatoxins, sterigmatocystin, A- and B-trichothecenes, zearalenone and its analogues, ochratoxin A, fumonisins, toxins, enniatins and beauvericin, moniliformin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin were prepared in RP-HPLC solvents and stored at -18 °C for 14 months. UV-spectroscopy was utilized to monitor the stability of analytes, excluding fumonisins. The gradual degradation of α-, β-zearalenol and α-, β-zearalanol in acetonitrile was detected. Aflatoxins and sterigmatocystin, zearalenone, toxins, enniatins and beauvericin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin can be referred to as stable. The concentration of the majority of trichothecenes should be monitored. Diluted multi-mycotoxin standard in water/methanol (50/50 ) solutions acidified with 0.1% formic acid proved to be stable in silanized glass at 23 °C exposed to light for at least 75 h (CV≤10%). An unexpected manifestation of MS/MS signal suppression/enhancement was discovered in the course of multi-mycotoxin standard solution stability evaluation.
标准溶液的真菌毒素在反相高效液相色谱溶剂中从纯标准品通常用于分析方法的开发。多真菌毒素高效液相色谱-串联质谱法需要对广泛的真菌代谢物进行稳定性评估。在标准储存和分析条件下，评估了真菌毒素在反相高效液相色谱溶剂和多分析物高效液相色谱-串联质谱校准品中的单个稀释储备标准溶液的稳定性。黄曲霉毒素、桔青霉素、A 和 B 型单端孢霉烯族化合物、玉米赤霉烯酮及其类似物、呕吐毒素、伏马菌素、交链孢霉毒素、恩镰孢菌素和 beauvericin、单端孢霉烯族化合物、桔青霉素、环匹罗嗪酸和桔青霉素、 citreoviridin 在反相高效液相色谱溶剂中制备，并储存在-18°C 下 14 个月。利用紫外光谱法监测除伏马菌素外的分析物的稳定性。在乙腈中检测到α-、β-玉米赤醇和α-、β-玉米赤烯醇的逐渐降解。黄曲霉毒素和桔青霉素、玉米赤霉烯酮、呕吐毒素、交链孢霉毒素、恩镰孢菌素和 beauvericin、桔青霉素、环匹罗嗪酸和 citreoviridin 可视为稳定。大多数单端孢霉烯族化合物的浓度应进行监测。用 0.1%甲酸酸化的水/甲醇（50/50）溶液稀释的多真菌毒素标准品在 23°C 下暴露于光下至少 75 小时（CV≤10%）时，在硅烷化玻璃中稳定。在多真菌毒素标准溶液稳定性评估过程中发现了意想不到的 MS/MS 信号抑制/增强表现。
