Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, China.
Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Oxid Med Cell Longev. 2020 Feb 10;2020:8569237. doi: 10.1155/2020/8569237. eCollection 2020.
The flavonoids were extracted from alfalfa using ethanol assisted with ultrasonic extraction and purified by D101 macroporous resin column chromatography. The chemical composition and content of ethanol elution fractions (EEFs) were assessed by ultrahigh-performance liquid chromatography and hybrid quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS) and aluminum nitrate-sodium nitrite-sodium hydroxide colorimetric method. The antioxidant activity of two EEFs was conducted by scavenging DPPH free radical, and the main antioxidants of 75% EEFs were screened using DPPH-UHPLC. Moreover, the antioxidant activity of 75% EEFs and the growth performance of broilers were studied. The results showed that the content of 30% and 75% EEFs was 26.20% and 62.57%. Fifteen compounds were identified from 75% EEFs, and five of them were reported in alfalfa for the first time. The scavenging activity of 75% and 30% EEFs (200 g/mL) against DPPH was 95.51% and 78.85%. The peak area of 5,3',4'-trihydroxyflavone and hyperoside was decreased by 82.69% and 76.04%, which exhibited strong scavenging capacities. The total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) level of three treated groups against the normal control group (NC) fed with basal diet significantly increased by 3.89-24.49%, 0.53-7.39%, and 0.79-11.79%, respectively. While the malondialdehyde (MDA) decreased by 0.47-18.27%. Compared with the NC, the feed to gain ratio (F : G) of three treated groups was lowered by 2.98-16.53% and survival rate of broilers significantly increased. Consequently, 75% EEFs extracted from alfalfa exhibited powerful antioxidant activities and might be a potential feed additive to poultry and livestock.
从苜蓿中用乙醇辅助超声提取提取了类黄酮，并通过 D101 大孔树脂柱色谱进行了纯化。通过超高效液相色谱和混合四极杆飞行时间质谱（UHPLC-Q-TOF-MS）和硝酸铝-亚硝酸钠-氢氧化钠比色法评估乙醇洗脱部分（EEF）的化学成分和含量。通过清除 DPPH 自由基对两种 EEF 的抗氧化活性进行了测试，并使用 DPPH-UHPLC 筛选了 75%EEF 的主要抗氧化剂。此外，还研究了 75%EEF 的抗氧化活性和肉鸡的生长性能。结果表明，30%和 75%EEF 的含量分别为 26.20%和 62.57%。从 75%EEF 中鉴定出 15 种化合物，其中 5 种是苜蓿中首次报道的。75%和 30%EEF（200μg/mL）对 DPPH 的清除活性分别为 95.51%和 78.85%。5,3',4'-三羟基黄酮和金丝桃苷的峰面积分别降低了 82.69%和 76.04%，表现出很强的清除能力。与基础日粮喂养的正常对照组（NC）相比，三个处理组的总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）水平分别显著提高了 3.89-24.49%、0.53-7.39%和 0.79-11.79%，而丙二醛（MDA）则降低了 0.47-18.27%。与 NC 相比，三个处理组的饲料增重比（F:G）降低了 2.98-16.53%，肉鸡的存活率显著提高。因此，从苜蓿中提取的 75%EEF 表现出强大的抗氧化活性，可能是家禽和家畜的一种潜在饲料添加剂。
