Chen Y M, Zhu Y, Lin E C
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Mol Gen Genet. 1987 Dec;210(2):331-7. doi: 10.1007/BF00325702.
In Escherichia coli the six known genes specifying the utilization of L-fucose as carbon and energy source cluster at 60.2 min and constitute a regulon. These genes include fucP (encoding L-fucose permease), fucI (encoding L-fucose isomerase), fucK (encoding L-fuculose kinase), fucA (encoding L-fuculose 1-phosphate aldolase), fucO (encoding L-1,2-propanediol oxidoreductase), and fucR (encoding the regulatory protein). In this study the fuc genes were cloned and their positions on the chromosome were established by restriction endonuclease and complementation analyses. Clockwise, the gene order is: fucO-fucA-fucP-fucI-fucK-fucR. The operons comprising the structural genes and the direction of transcription were determined by complementation analysis and Southern blot hybridization. The fucPIK and fucA operons are transcribed clockwise. The fucO operon is transcribed counterclockwise. The fucR gene product activates the three structural operons in trans.
在大肠杆菌中，已知的6个负责将L-岩藻糖作为碳源和能源利用的基因聚集在60.2分钟处，构成一个调节子。这些基因包括fucP（编码L-岩藻糖通透酶）、fucI（编码L-岩藻糖异构酶）、fucK（编码L-岩藻酮糖激酶）、fucA（编码L-岩藻酮糖1-磷酸醛缩酶）、fucO（编码L-1,2-丙二醇氧化还原酶）和fucR（编码调节蛋白）。在本研究中，fuc基因被克隆，并通过限制性内切酶和互补分析确定了它们在染色体上的位置。按顺时针方向，基因顺序为：fucO-fucA-fucP-fucI-fucK-fucR。通过互补分析和Southern印迹杂交确定了包含结构基因的操纵子及其转录方向。fucPIK和fucA操纵子按顺时针方向转录。fucO操纵子按逆时针方向转录。fucR基因产物可反式激活三个结构操纵子。
