Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Rwanda Institute of Conservation and Agriculture, Gashora, Bugesera, Rwanda.
J Gen Virol. 2021 Feb;102(2). doi: 10.1099/jgv.0.001523.
Bunyamwera (BUNV), Batai (BATV) and Ngari (NRIV) are mosquito-borne viruses that are members of the genus in the order Bunyavirales. These three viruses are enveloped with single-stranded, negative-sense RNA genomes consiting of three segments, denoted as Small (S), Medium (M) and Large (L). Ngari is thought to be the natural reassortant progeny of Bunyamwera and Batai viruses. The relationship between these 'parental' viruses and the 'progeny' poses an interesting question, especially given that there is overlap in their respective transmission ecologies, but differences in their infection host ranges and pathogenesis. We compared the kinetics of these three viruses in a common laboratory system and found no significant difference in growth kinetics. There was, however, a tendency of BATV to have smaller plaques than either BUNV or NRIV. Furthermore, we determined that all three viruses are stable in extracellular conditions and retain infectivity for a week in non-cellular media, which has public health and biosafety implications. The study of this understudied group of viruses addresses a need for basic characterization of viruses that have not yet reached epidemic transmission intensity, but that have the potential due to their infectivity to both human and animal hosts. These results lay the groundwork for future studies of these neglected viruses of potential public and One Health importance.
布尼亚病毒（BUNV）、巴泰病毒（BATV）和尼帕病毒（NRIV）是通过蚊子传播的病毒，属于布尼亚病毒目布尼亚病毒科的正布尼亚病毒属。这三种病毒均为包膜单股负链 RNA 病毒，由三个节段组成，分别命名为小（S）、中（M）和大（L）。尼帕病毒被认为是布尼亚病毒和巴泰病毒的自然重组子代。这些“亲代”病毒和“子代”之间的关系提出了一个有趣的问题，特别是考虑到它们在各自的传播生态中有重叠，但在感染宿主范围和发病机制上存在差异。我们在一个常见的实验室系统中比较了这三种病毒的动力学特性，发现它们的生长动力学没有显著差异。然而，BATV 形成的噬菌斑倾向于比 BUNV 或 NRIV 更小。此外，我们还确定这三种病毒在细胞外环境中均稳定，在非细胞培养基中可保持一周的感染性，这对公共卫生和生物安全具有重要意义。对这组研究较少的病毒进行研究，是为了满足对尚未达到流行传播强度但由于其对人类和动物宿主的感染性而具有潜在传播能力的病毒进行基本特征描述的需要。这些结果为未来对这些具有潜在公共卫生和“同一健康”重要性的被忽视病毒的研究奠定了基础。
