Department of Marine and Coastal Sciences, Rutgers, the State University of New Jersey, New Brunswick, NJ 08901, USA.
FEMS Microbiol Ecol. 2021 Mar 10;97(3). doi: 10.1093/femsec/fiab001.
This minireview will discuss the improvements in Oxford Nanopore (Oxford; sequencing technology that make the MinION a viable platform for microbial ecology studies. Specific issues being addressed are the increase in sequence accuracy from 65 to 96.5% during the last 5 years, the ability to obtain a quantifiable/predictive signal from the MinION with respect to target molecule abundance, simple-to-use GUI-based pathways for data analysis and the modest additional equipment needs for sequencing in the field. Coupling these recent improvements with the low capital costs for equipment and the reasonable per sample cost makes MinION sequencing an attractive option for virtually any laboratory.
本文将讨论牛津纳米孔（Oxford；测序技术在过去 5 年中取得的进展，这些进展使得 MinION 成为微生物生态学研究的可行平台。文中具体讨论了几个问题，包括序列准确性从 65%提高到 96.5%，MinION 能够针对目标分子丰度产生可量化/可预测的信号，基于图形用户界面的简单数据分析方法，以及在现场测序所需的适度额外设备需求。将这些最新进展与设备的低资本成本和合理的每个样本成本相结合，使得 MinION 测序成为几乎任何实验室的一个有吸引力的选择。
