Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, Institute of Advanced Materials Physics, School of Sciences, Tianjin University, Tianjin, 300350, China.
Department of Neurosurgery and Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Tianjin Medical University General Hospital, Tianjin 300052, China.
Theranostics. 2021 Jan 1;11(6):2806-2821. doi: 10.7150/thno.51912. eCollection 2021.
Traumatic brain injury (TBI) is a sudden injury to the brain, accompanied by the production of large amounts of reactive oxygen and nitrogen species (RONS) and acute neuroinflammation responses. Although traditional pharmacotherapy can effectively decrease the immune response of neuron cells via scavenging free radicals, it always involves in short reaction time as well as rigorous clinical trial. Therefore, a noninvasive topical treatment method that effectively eliminates free radicals still needs further investigation. In this study, a type of catalytic patch based on nanozymes with the excellent multienzyme-like activity is designed for noninvasive treatment of TBI. The enzyme-like activity, free radical scavenging ability and therapeutic efficacy of the designed catalytic patch were assessed and . The structural composition was characterized by the X-ray diffraction, X-ray photoelectron spectroscopy and high-resolution transmission electron microscopy technology. Herein, the prepared Cr-doped CeO (Cr/CeO) nanozyme increases the reduced Ce states, resulting in its enzyme-like activity 3-5 times higher than undoped CeO. Furthermore, Cr/CeO nanozyme can improve the survival rate of LPS induced neuron cells via decreasing excessive RONS. The experiments show the Cr/CeO nanozyme can promote wound healing and reduce neuroinflammation of mice following brain trauma. The catalytic patch based on nanozyme provides a noninvasive topical treatment route for TBI as well as other traumas diseases. The catalytic patch based on nanozyme provides a noninvasive topical treatment route for TBI as well as other traumas diseases.
创伤性脑损伤（TBI）是一种突发的脑部损伤，伴随着大量活性氧和氮物种（RONS）的产生和急性神经炎症反应。虽然传统的药物治疗可以通过清除自由基有效地降低神经元细胞的免疫反应，但它总是涉及到短的反应时间和严格的临床试验。因此，一种有效的消除自由基的非侵入性局部治疗方法仍然需要进一步研究。在这项研究中，设计了一种基于纳米酶的催化贴片，用于 TBI 的非侵入性治疗。评估了设计的催化贴片的酶样活性、自由基清除能力和治疗效果。采用 X 射线衍射、X 射线光电子能谱和高分辨率透射电子显微镜技术对结构组成进行了表征。在此，制备的 Cr 掺杂 CeO（Cr/CeO）纳米酶增加了还原态的 Ce，使其酶样活性比未掺杂的 CeO 高 3-5 倍。此外，Cr/CeO 纳米酶可以通过减少过量的 RONS 来提高 LPS 诱导的神经元细胞的存活率。实验表明，Cr/CeO 纳米酶可以促进脑创伤后小鼠的伤口愈合和减少神经炎症。基于纳米酶的催化贴片为 TBI 及其他创伤性疾病提供了一种非侵入性的局部治疗途径。
