Medical and Clinical Genetics, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Food and Nutrition, University of Helsinki, Helsinki, Finland.
Metabolomics. 2021 Jan 29;17(2):20. doi: 10.1007/s11306-020-01752-5.
Maternal metabolism changes substantially during pregnancy. However, few studies have used metabolomics technologies to characterize changes across gestation.
We applied liquid chromatography-mass spectrometry (LC-MS) based non-targeted metabolomics to determine whether the metabolic profile of serum differs throughout the pregnancy between pre-eclamptic and healthy women in the FINNPEC (Finnish Genetics of Preeclampsia Consortium) Study. Serum samples were available from early and late pregnancy.
Progression of pregnancy had large-scale effects to the serum metabolite profile. Altogether 50 identified metabolites increased and 49 metabolites decreased when samples of early pregnancy were compared to samples of late pregnancy. The metabolic signatures of pregnancy were largely shared in pre-eclamptic and healthy women, only urea, monoacylglyceride 18:1 and glycerophosphocholine were identified to be increased in the pre-eclamptic women when compared to healthy controls.
Our study highlights the need of large-scale longitudinal metabolomic studies in non-complicated pregnancies before more detailed understanding of metabolism in adverse outcomes could be provided. Our findings are one of the first steps for a broader metabolic understanding of the physiological changes caused by pregnancy per se.
孕妇的新陈代谢在怀孕期间会发生重大变化。然而，很少有研究使用代谢组学技术来描述整个妊娠期间的变化。
我们应用基于液相色谱-质谱（LC-MS）的非靶向代谢组学方法，来确定在 FINNPEC（芬兰子痫前期遗传学研究联盟）研究中，子痫前期患者和健康孕妇的血清代谢谱在整个孕期是否存在差异。血清样本可取自早孕期和晚孕期。
妊娠进展对血清代谢物谱有较大影响。与早孕期样本相比，晚孕期样本中共有 50 种鉴定出的代谢物增加，49 种代谢物减少。子痫前期患者和健康孕妇的妊娠代谢特征基本相同，与健康对照组相比，仅发现尿素、单酰基甘油 18:1 和甘油磷酸胆碱在子痫前期患者中增加。
本研究强调在更详细地了解不良妊娠结局中的代谢变化之前，需要对非复杂妊娠进行大规模的纵向代谢组学研究。我们的研究结果是对妊娠本身引起的生理变化进行更广泛代谢理解的初步步骤之一。
