College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
Arch Microbiol. 2021 Aug;203(6):3117-3124. doi: 10.1007/s00203-021-02303-9. Epub 2021 Apr 2.
A bacterial strain ODT-83 is isolated from oysters, which is capable of adsorbing norovirus (NoV) via histo-blood group antigen-like (HBGA-like) substances. To better understand its genetic background associated with the production of HBGA-like substances, the genome of the ODT-83 was completely sequenced and analyzed. The ODT-83 only contains one circular chromosome, with a length of 5,384,159 bp. Both the 16S rRNA gene phylogeny and the average nucleotide identity (ANI) analyses confirm that the ODT-83 is a new Pseudomonas oleovorans strain. The whole genome encodes a total of 5037 predicted open reading frames (ORFs), 66 tRNA genes and 12 rRNA genes. Two gene clusters are detected on the genome, which are involved in the synthesis of polysaccharides of alginate and Pel, respectively. These results lay the foundation for further research on the interaction between the P. oleovorans strain ODT-83 and NoV.
从牡蛎中分离到一株能够通过类组织血型抗原（HBGA-like）物质吸附诺如病毒（NoV）的细菌菌株 ODT-83。为了更好地了解其与 HBGA-like 物质产生相关的遗传背景，对 ODT-83 的基因组进行了完全测序和分析。ODT-83 仅包含一条长度为 5,384,159bp 的环状染色体。16S rRNA 基因系统发育分析和平均核苷酸同一性（ANI）分析均证实 ODT-83 是一株新的解油假单胞菌。全基因组共编码 5037 个预测的开放阅读框（ORFs）、66 个 tRNA 基因和 12 个 rRNA 基因。在基因组上检测到两个基因簇，分别涉及海藻酸盐和 Pel 多糖的合成。这些结果为进一步研究 ODT-83 与 NoV 之间的相互作用奠定了基础。
