Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.
Plant Pathology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.
Sci Rep. 2021 Jun 18;11(1):12876. doi: 10.1038/s41598-021-92171-6.
Bacterial metal reducers were isolated from water samples collected from harsh condition locations in Egypt. Four selected isolates were identified as Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculata for Copper (Cu), Iron (Fe), Cobalt (Co) and Zinc (Zn) Nanoparticles (NPs) production sequentially. Nitrate reductase enzyme was assayed for bacterial isolates which demonstrated that P. putida, and M. hydrocarbonoclasticus have the maximum enzyme production. The produced NPs were characterized by using XRD, TEM, UV-VIS spectroscopy. Magnetic properties for all selected metals NPs were measured using Vibrating Sample Magnetometer (VSM) and demonstrated that FeNPs recorded the highest magnetization value. The antibacterial activity of selected metals NPs was tested against some phytopathogenic bacteria causing the following diseases: soft rot (Pectobacterium carotovorum, Enterobacter cloacae), blackleg (Pectobacterium atrosepticum and Dickeya solani), brown rot (Ralstonia solanacearum), fire blight (Erwinia amylovora) and crown gall (Agrobacterium tumefaciens). All metals NPs showed an antagonistic effect against the tested isolates, particularly, FeNPs showed the highest antibacterial activity followed by CuNPs, and ZnNPs. Due to the small size, high reactivity, and large surface area of biologically synthesized NPs, they are used as a good disinfector, and can be considered as a new and alternative approach to traditional disease management methods.
从埃及恶劣条件地区采集的水样中分离出了细菌金属还原剂。 从水样中分离出了四种细菌，分别为屎肠球菌、铜绿假单胞菌、海油烃球菌和弯杆菌，用于顺序生产铜 (Cu)、铁 (Fe)、钴 (Co) 和锌 (Zn) 纳米颗粒 (NPs)。 对细菌分离物进行了硝酸还原酶酶的测定，结果表明铜绿假单胞菌和海油烃球菌具有最大的酶产量。 使用 XRD、TEM、UV-VIS 光谱对所制备的 NPs 进行了表征。 使用振动样品磁强计 (VSM) 测量了所有选定金属 NPs 的磁性，结果表明 FeNPs 记录的磁化值最高。 对选定金属 NPs 的抗菌活性进行了测试，以评估它们对引起以下疾病的一些植物病原菌的抑制效果：软腐病（果胶杆菌、阴沟肠杆菌）、黑胫病（果胶杆菌和迪基叶杆菌）、褐腐病（茄科雷尔氏菌）、火疫病（欧文氏菌）和冠瘿病（根癌农杆菌）。 所有金属 NPs 都对测试的分离物表现出拮抗作用，特别是 FeNPs 表现出最高的抗菌活性，其次是 CuNPs 和 ZnNPs。 由于生物合成 NPs 具有尺寸小、反应性高、比表面积大等特点，它们可用作良好的消毒剂，并且可以被认为是传统疾病管理方法的一种新的替代方法。
