Zhang Pengfei, Wang Shaopeng
Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, 85287, USA.
School of Biological and Health Systems Engineering, Arizona State University, Tempe, 85287, USA.
Biocell. 2021 Sep 1;45(6):1449-1451. doi: 10.32604/biocell.2021.017607.
The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity.
外泌体介导的反应可通过调节细胞内信号通路来促进或抑制疾病，这使得外泌体成为单细胞水平诊断和治疗控制的有效标志物。然而，传统方法难以实现实时分析，因为外泌体通常需要通过超速离心进行富集以获得可测量的信噪比。最近开发的无标记单分子成像方法由于其极高的灵敏度，可能成为分析单个外泌体及单个活细胞相关分泌行为的实时定量工具。
