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两种用于检测沙眼衣原体抗体的免疫荧光试验的比较。

Comparison of two immunofluorescence tests for detecting antibodies to C. trachomatis.

作者信息

Forsey T, Stainsby K, Hoger P H, Ridgway G L, Darougar S, Fischer-Brugge U

出版信息

Eur J Epidemiol. 1986 Jun;2(2):163-4. doi: 10.1007/BF00157029.

DOI:10.1007/BF00157029
PMID:3533611
Abstract

Two immunofluorescence tests were compared for detecting antibodies to chlamydiae. The inclusion antigen test was more sensitive, detecting antibodies in more sera and at higher titres. The micro-IF test was more specific, differentiating between antibodies to C. trachomatis and those to C.IOL 207. Antibodies to this non-genital chlamydial type accounted for half the positive results. These antibodies can cause the prevalence of C. trachomatis infections to be over estimated when genus-specific serology tests are used.

摘要

比较了两种免疫荧光试验检测衣原体抗体的效果。包涵体抗原试验更敏感，能在更多血清中检测到抗体，且抗体滴度更高。微量免疫荧光试验更具特异性，能区分沙眼衣原体抗体和鹦鹉热衣原体IOL 207抗体。针对这种非生殖道衣原体类型的抗体占阳性结果的一半。当使用属特异性血清学检测时，这些抗体会导致沙眼衣原体感染的流行率被高估。

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引用本文的文献

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J Clin Microbiol. 1994 Jun;32(6):1419-26. doi: 10.1128/jcm.32.6.1419-1426.1994.
2
Antibodies to Chlamydia trachomatis.沙眼衣原体抗体。
Genitourin Med. 1987 Aug;63(4):279. doi: 10.1136/sti.63.4.279.
3
Comparison between cell culture and serology for detecting Chlamydia trachomatis in women seeking abortion.细胞培养法与血清学检测法在检测寻求堕胎女性沙眼衣原体中的比较。

本文引用的文献

1
Prevalence of antichlamydial antibody in London blood donors.伦敦献血者中抗衣原体抗体的流行情况。
Br J Vener Dis. 1980 Dec;56(6):404-7. doi: 10.1136/sti.56.6.404.
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Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test.新型微量滴定间接免疫荧光试验中生殖系统沙眼衣原体、性病性淋巴肉芽肿及相关病原体之间的免疫学关系
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Chlamydia specific IgG and IgA antibodies in women with obstructive infertility as determined by immunoblotting and immunoperoxidase assays.通过免疫印迹法和免疫过氧化物酶测定法测定的阻塞性不孕症女性中的衣原体特异性IgG和IgA抗体。
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Comparison of immunofluorescence tests for detecting antibodies to Chlamydia trachomatis.检测沙眼衣原体抗体的免疫荧光试验比较
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Detecting Chlamydia trachomatis in men with urethritis: serology v isolation in cell culture.检测尿道炎男性中的沙眼衣原体：血清学检测与细胞培养分离法的比较
Genitourin Med. 1989 Jun;65(3):166-70. doi: 10.1136/sti.65.3.166.
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Laboratory diagnosis of human chlamydial infections.人类衣原体感染的实验室诊断
Clin Microbiol Rev. 1989 Apr;2(2):119-36. doi: 10.1128/CMR.2.2.119.
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Enzyme immunoassay to determine exposure to Chlamydia pneumoniae (strain TWAR).用于确定肺炎衣原体（TWAR 菌株）暴露情况的酶免疫测定法。
J Clin Microbiol. 1989 Dec;27(12):2778-83. doi: 10.1128/jcm.27.12.2778-2783.1989.
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Current problems in microbiology: 1. Chlamydial infections: which laboratory test?微生物学当前的问题：1. 衣原体感染：采用哪种实验室检测方法？
J Clin Pathol. 1991 Jan;44(1):1-5. doi: 10.1136/jcp.44.1.1.
10
Chlamydiae as pathogens--an overview of diagnostic techniques, clinical features, and therapy of human infections.衣原体作为病原体——人类感染的诊断技术、临床特征及治疗概述
Klin Wochenschr. 1991 Aug 1;69(11):463-73. doi: 10.1007/BF01649417.
冈比亚儿童对沙眼的血清及结膜抗体反应。
J Hyg (Lond). 1972 Dec;70(4):727-40. doi: 10.1017/s0022172400022579.
4
Prevalence in human beings of antibodies to Chlamydia IOL-207, an atypical strain of chlamydia.人类中针对衣原体非典型菌株衣原体IOL-207抗体的流行情况。
J Infect. 1986 Mar;12(2):145-52. doi: 10.1016/s0163-4453(86)93608-x.
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Fluorescent antibody studies in chlamydial infections.衣原体感染中的荧光抗体研究。
J Clin Microbiol. 1975 Apr;1(4):345-52. doi: 10.1128/jcm.1.4.345-352.1975.
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Single-antigen immunofluorescence test for chlamydial antibodies.衣原体抗体的单抗原免疫荧光检测
J Clin Microbiol. 1978 Aug;8(2):119-22. doi: 10.1128/jcm.8.2.119-122.1978.
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Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.改良微量免疫荧光试验以提供衣原体感染的常规血清学诊断检测。
J Clin Pathol. 1977 Jun;30(6):510-7. doi: 10.1136/jcp.30.6.510.