Gambino Delia, Gargano Valeria, Butera Gaspare, Sciortino Sonia, Pizzo Mariangela, Oliveri Giuseppa, Cardamone Cinzia, Piraino Chiara, Cassata Giovanni, Vicari Domenico, Costa Antonella
Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.
Microorganisms. 2022 Apr 6;10(4):780. doi: 10.3390/microorganisms10040780.
spp. are among the most frequent causes of foodborne diseases, and the increasing occurrence of MDR strains is an additional cause for concern. In the three-year period 2019-2021, we collected spp. strains isolated from different food categories analysed in the context of Regulation (EC) No 2073/2005 in order to assess their antibiotic susceptibility profiles and ESBL production. To determine the susceptibility profiles and identify MDR strains, we used the Kirby-Bauer method to test 17 antibiotics. Double-disc and PCR testing then allowed us to assess the production of ESBLs and the presence of beta-lactamase resistance genes. Phenotypic tests showed that 36 out of 67 strains were MDR and 52.7% of these were ESBL producers. Finally, molecular investigations conducted on ESBL-producing strains revealed the presence of , and genes. Our results confirmed the prevalence of , an MDR strain and ESBL producer, in chicken meat. This suggests that further research on the prevalence of antibiotic resistance genes (ARGs) in foodborne strains is needed, especially from a One Health perspective.
某些物种是食源性疾病最常见的病因之一，多重耐药菌株的不断出现更是令人担忧。在2019年至2021年的三年期间，我们收集了在第2073/2005号（欧盟）法规框架下分析的不同食品类别中分离出的某些物种菌株，以评估它们的抗生素敏感性谱和超广谱β-内酰胺酶（ESBL）产生情况。为了确定敏感性谱并鉴定多重耐药菌株，我们使用纸片扩散法测试了17种抗生素。然后通过双纸片法和聚合酶链反应（PCR）测试来评估ESBL的产生情况以及β-内酰胺酶耐药基因的存在情况。表型测试表明，67株菌株中有36株为多重耐药，其中52.7%产生ESBL。最后，对产生ESBL的菌株进行的分子研究揭示了某些基因的存在。我们的结果证实了某多重耐药菌株和ESBL产生菌在鸡肉中的流行情况。这表明需要进一步研究食源菌株中抗生素耐药基因（ARGs）的流行情况，特别是从“同一健康”的角度。
