In most regions of the world, tuberculosis (TB) is classified as a malignant infectious disease that can be fatal. Using advanced tools and technology, automatic analysis and classification of chest X-rays (CXRs) into TB and non-TB can be a reliable alternative to the subjective assessment performed by healthcare professionals. Thus, in the study, we propose an automatic TB detection system using advanced deep learning (DL) models. A significant portion of a CXR image is dark, providing no information for diagnosis and potentially confusing DL models. Therefore, in the proposed system, we use sophisticated segmentation networks to extract the region of interest from multimedia CXRs. Then, segmented images are fed into the DL models. For the subjective assessment, we use explainable artificial intelligence to visualize TB-infected parts of the lung. We use different convolutional neural network (CNN) models in our experiments and compare their classification performance using three publicly available CXR datasets. EfficientNetB3, one of the CNN models, achieves the highest accuracy of 99.1%, with a receiver operating characteristic of 99.9%, and an average accuracy of 98.7%. Experiment results confirm that using segmented lung CXR images produces better performance than does using raw lung CXR images.