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根癌农杆菌介导的单子叶唐菖蒲属植物转化：T-DNA编码基因表达的检测

Agrobacterium tumefaciens-mediated transformation of the monocot genus Gladiolus: detection of expression of T-DNA-encoded genes.

作者信息

Graves A C, Goldman S L

出版信息

J Bacteriol. 1987 Apr;169(4):1745-6. doi: 10.1128/jb.169.4.1745-1746.1987.

DOI:10.1128/jb.169.4.1745-1746.1987
PMID:3558323
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212009/
Abstract

Agrobacterium tumefaciens was capable of directing the transformation of Gladiolus sp., a monocot genus belonging to the family Iridaceae. Only strains capable of transferring T-DNA formed tumors, sections of which could be cultured in phytohormone-free media. Opine synthase activities were also observed in homogenates made from these tumors.

摘要

根癌土壤杆菌能够介导鸢尾科单子叶植物唐菖蒲属的转化。只有能够转移T-DNA的菌株才能形成肿瘤，这些肿瘤的切片可以在无植物激素的培养基中培养。在由这些肿瘤制成的匀浆中也观察到了冠瘿碱合成酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f9/212009/93f5e69909bb/jbacter00194-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f9/212009/93f5e69909bb/jbacter00194-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f9/212009/93f5e69909bb/jbacter00194-0391-a.jpg

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本文引用的文献

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EMBO J. 1984 Dec 20;3(13):3039-41. doi: 10.1002/j.1460-2075.1984.tb02254.x.
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VirA, the plant-signal receptor, is responsible for the Ti plasmid-specific transfer of DNA to maize by Agrobacterium.VirA作为植物信号受体，负责农杆菌将DNA特异性转移至玉米的Ti质粒。
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