Song Xiaoxia, Fu Wei, Cheang U Kei
Department of Mechanical and Energy Engineering, Southern University of Science and Technology, Shenzhen 518055, China.
Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems, Southern University of Science and Technology, Shenzhen, China.
iScience. 2022 Jun 2;25(7):104507. doi: 10.1016/j.isci.2022.104507. eCollection 2022 Jul 15.
To realize the potential to use micro/nanorobots for targeted cancer therapy, it is important to improve their biocompatibility and targeting ability. Here, we report on drug-loaded magnetic microrobots capable of polarizing macrophages into the antitumor phenotype to target and inhibit cancer cells. tests demonstrated that the microrobots have good biocompatibility with normal cells and immune cells. Positively charged DOX was loaded onto the surface of microrobots via electrostatic interactions and exhibited pH-responsive release behavior. The nano-smooth surfaces of the microrobots activated M1 polarization of macrophages, thus activating their intrinsic targeting and antitumor abilities toward cancer cells. Through dual targeting from magnetic guidance and M1 macrophages, the microrobots were able to target and kill cancer cells in a 3D tumor spheroid culture assay. These findings demonstrate a way to improve the tumor-targeting and antitumor abilities of microrobots through the combined use of magnetic control, macrophages, and pH-responsive drug release.
为了实现使用微型/纳米机器人进行靶向癌症治疗的潜力，提高它们的生物相容性和靶向能力很重要。在此，我们报道了一种载药磁性微型机器人，它能够将巨噬细胞极化为抗肿瘤表型，以靶向和抑制癌细胞。测试表明，这些微型机器人与正常细胞和免疫细胞具有良好的生物相容性。带正电荷的阿霉素通过静电相互作用负载在微型机器人表面，并表现出pH响应释放行为。微型机器人的纳米光滑表面激活了巨噬细胞的M1极化，从而激活了它们对癌细胞的内在靶向和抗肿瘤能力。通过磁导向和M1巨噬细胞的双重靶向，这些微型机器人能够在三维肿瘤球状体培养试验中靶向并杀死癌细胞。这些发现展示了一种通过联合使用磁控、巨噬细胞和pH响应药物释放来提高微型机器人肿瘤靶向和抗肿瘤能力的方法。
