Department of Veterinary Medicine, Hessian State Laboratory (LHL), Giessen, Germany.
Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005454.
Seven genotypically distinct strains assigned to the genus were isolated in different laboratories from several animal sources. Strain D17_0559-3-2-1 and three further strains were isolated from samples of duck, pig and goose. The strains had >99 % 16S rRNA gene sequence similarity to each other and to strain VA92-K48 and two further strains isolated from samples of medical leech and a turtle. The closest related type strains to the seven strains were those of (96.74 %) and (95.93 %). Average nucleotide identity, amino acid identity and DNA-DNA hybridization results showed that the strains represented two separate novel species. One further phylogenetically distinct strain (165301687) was isolated from fox urine. The strain had highest 16S rRNA gene sequence similarity to the type strains of (95.67 %), followed by (95.58 %) and (94.22 %) and represented a further novel species. Chemotaxonomic and physiological data of the novel strains were assessed, but failed to unequivocally differentiate the novel species from existing members of the genus. MALDI-TOF MS data proved the discrimination of at least strain 165301687 from all currently described species. Based on the presented phylogenomic and physiological data, we propose three novel species, sp. nov. with strain D17_0559-3-2-1 (=DSM 111258= CIP 111884=CCM 9044) as type strain, sp. nov. with strain VA92-K48 (=DSM 106012=LMG 30351=CIP 111492) as type strain and sp. nov. with strain 165301687 (=DSM 106013= LMG 30352= CIP 111494) as type strain.
从不同动物来源的多个样本中,在不同实验室分离到了七个基因型不同的归属于气单胞菌属的菌株。菌株 D17_0559-3-2-1 和另外三个菌株从鸭子、猪和鹅的样本中分离而来。这些菌株之间的 16S rRNA 基因序列相似性>99%,与菌株 VA92-K48 以及从医用水蛭和龟的样本中分离到的另外两个菌株也相似。这 7 个菌株最接近的参考菌株是 (96.74%)和 (95.93%)。平均核苷酸同一性、氨基酸同一性和 DNA-DNA 杂交结果表明,这些菌株代表了两个独立的新种。从狐狸尿液中分离到的另一个系统发育上不同的菌株(165301687)。该菌株与气单胞菌属的模式菌株的 16S rRNA 基因序列相似性最高,为 95.67%,其次为 (95.58%)和 (94.22%),代表了另一个新种。对新菌株的化学生物和生理数据进行了评估,但未能明确区分新种与属内现有种。MALDI-TOF MS 数据证明了至少菌株 165301687 与所有目前描述的种都可区分。基于呈现的系统基因组和生理数据,我们提出了三个新种,即 sp. nov.,以 D17_0559-3-2-1 株(=DSM 111258=CIP 111884=CCM 9044)为模式株, sp. nov.,以 VA92-K48 株(=DSM 106012=LMG 30351=CIP 111492)为模式株,以及 sp. nov.,以 165301687 株(=DSM 106013=LMG 30352=CIP 111494)为模式株。