Dashora Alankrita, Rathore Kavita, Raj Shani, Sharma Kanika
Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, India.
Biochem Biophys Rep. 2022 Aug 12;31:101320. doi: 10.1016/j.bbrep.2022.101320. eCollection 2022 Sep.
The mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV-Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used to characterize the produced AgNPs. UV-Vis analysis showed a characteristic peak at 435 nm corresponding to surface plasmon resonance. The synthesis process was spectrophotometrically optimized for various parameters. After optimization, highly stable AgNPs were prepared using 3.0 ml of leaf extract, pH 7.0, 1.0 mM AgNO, and 60 °C. The zeta potential was measured by DLS, which showed -20.8 mV and the PDI value was 5.42. TEM and SEM analysis shows a spherical shape of the synthesized nanoparticles, and the size was measured between 10 and 40 nm. EDX analysis showed intense peaks from silver and oxygen and small peaks from various metal atoms such as Na, P, S and Al indicating their presence in trace amounts. The average size of the PL-AgNPs was 14 nm. The phytochemical analysis shows that the presence of alkaloids, essential oils and saponins seems to be responsible for the synthesis of nanoparticles. PL-AgNPs were further investigated for their antifungal activity against . The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and effect of nanoparticles on cytomorphology of have also been reported. Biosynthesized nanoparticles have proven to be inexpensive, environmentally friendly, stable, easily reproducible, and highly effective against plant-pathogenic fungi.
植物介导的银（PL-AgNPs）纳米颗粒非常稳定且高效。采用紫外-可见光谱、动态光散射（DLS）、X射线衍射（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）对所制备的AgNPs进行表征。紫外-可见分析显示在435nm处有一个对应于表面等离子体共振的特征峰。对合成过程的各种参数进行了分光光度法优化。优化后，使用3.0ml叶提取物、pH 7.0、1.0mM硝酸银和60°C制备了高度稳定的AgNPs。通过DLS测量zeta电位，结果显示为-20.8mV，PDI值为5.42。TEM和SEM分析表明合成的纳米颗粒呈球形，尺寸在10至40nm之间。EDX分析显示银和氧的峰很强，而钠、磷、硫和铝等各种金属原子的峰较小，表明它们以痕量存在。PL-AgNPs的平均尺寸为14nm。植物化学分析表明，生物碱、精油和皂苷的存在似乎是纳米颗粒合成的原因。进一步研究了PL-AgNPs对……的抗真菌活性。还报道了最小抑菌浓度（MIC）、最小杀菌浓度（MFC）以及纳米颗粒对……细胞形态的影响。生物合成的纳米颗粒已被证明价格低廉、环境友好、稳定、易于重现且对植物病原真菌高效。
