常规痰液细菌学中肺炎球菌抗原快速检测方法的评估
Assessment of rapid methods of pneumococcal antigen detection in routine sputum bacteriology.
作者信息
Whitby M, Kristinsson K G, Brown M
出版信息
J Clin Pathol. 1985 Mar;38(3):341-4. doi: 10.1136/jcp.38.3.341.
Abstract
Sputum specimens from 480 patients were examined for the presence of pneumococci by Gram film and culture and for pneumococcal antigen by counterimmunoelectrophoresis, coagglutination, and latex agglutination. Ninety six positive specimens were detected. Gram film and culture provided the most reliable techniques in well taken specimens collected early in the illness before antibiotic treatment had started. More than 70% of the specimens examined were submitted after starting antibiotics, however, and in these specimens, methods of antigen detection proved of greater value than either Gram film or culture. Counterimmunoelectrophoresis, coagglutination, and latex agglutination were similar in sensitivity and specificity, but coagglutination and latex agglutination were much easier to perform and to read.
摘要
对480例患者的痰液标本进行了检查，通过革兰氏涂片和培养检测肺炎球菌的存在，并通过对流免疫电泳、协同凝集和乳胶凝集检测肺炎球菌抗原。检测到96份阳性标本。在疾病早期、抗生素治疗开始前采集的质量良好的标本中，革兰氏涂片和培养提供了最可靠的技术。然而，超过70%的检测标本是在开始使用抗生素后提交的，在这些标本中，抗原检测方法比革兰氏涂片或培养更有价值。对流免疫电泳、协同凝集和乳胶凝集在敏感性和特异性方面相似，但协同凝集和乳胶凝集操作和判读起来要容易得多。
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